DAG測定性能：

現(xiàn)成的試劑盒包括BacMam中的DAG傳感器，這是一種BSL-1病毒載體，可有效輸送到大多數(shù)細(xì)胞類型。純化的BacMam，AAV，Lenti載體可根據(jù)要求提供。

1、在讀板器或成像系統(tǒng)上容易檢測到響應(yīng)

2、一致的良好表達(dá)

3、高信噪比

艾美捷 Montana Molecular-DAG多重檢測試驗(yàn)：

單通道，非FRET信號

可提供紅色或綠色熒光的DAG（二酰基甘油）傳感器版本

通過結(jié)合紅色和綠色傳感器，可以同時(shí)測量多個(gè)GPCR信號通路。

Montana Molecular強(qiáng)大的多功能DAG檢測試劑盒：

實(shí)時(shí)檢測二酰基甘油信號

在活細(xì)胞中篩選與Gq蛋白偶聯(lián)的GPCRs

病毒載體傳遞，適用于幾乎所有細(xì)胞類型

靶向混合培養(yǎng)中的微域或特定細(xì)胞

簡單的操作流程：

z小化的液體處理

無需細(xì)胞裂解

兼容96孔或384孔板

為您選擇合適的傳感器：上升型還是下降型？

左圖：下降型DAG在二酰基甘油水平增加時(shí)，熒光強(qiáng)度從亮變暗。這種檢測方法在熒光板讀數(shù)器上容易被檢測到，有助于成像那些表達(dá)效率低的細(xì)胞。

右圖：上升型DAG在二酰基甘油水平增加時(shí)，熒光強(qiáng)度從暗變亮。這種DAG檢測方法在熒光板讀數(shù)器或成像系統(tǒng)上容易被檢測到。

文獻(xiàn)參考：

X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. bioRxiv. January 2024.

Z. Miller, et al. Lidocaine induces apoptosis in head and neck squamous cell carcinoma through activation of bitter taste receptor T2R14. Cell Reports. November 2023. (bioRxiv)

N. Za?mia, et al. GLP-1 and GIP receptors signal through distinct β-arrestin 2-dependent pathways to regulate pancreatic β cell function. Cell Reports. October 2023.

G. Sanchez, et al. Coincident Regulation of PLCβ Signaling by Gq-Coupled and μ-Opioid Receptors Opposes Opioid-Mediated Antinociception. Molecular Pharmacology. December 2022.

M. Doepner, et al. Endogenous DOPA inhibits melanoma through suppression of CHRM1 signaling. Science Advances. September 2022.

A. Kim, et al. Arginine-vasopressin mediates counter-regulatory glucagon release and is diminished in type 1 diabetes. eLife. November 2021. (bioRxiv)

S. Hoare, T. Hughes. Biosensor Assays for Measuring the Kinetics of G-Protein and Arrestin-Mediated Signaling in Live Cells. The Assay Guidance Manual. September 2021.

E. Maguire, et al. The Alzheimer's disease protective P522R variant of PLCG2, consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function. bioRxiv. April 2020.

L. Liu, et al. Diacylglycerol kinases regulate TRPV1 channel activity. Journal of Biological Chemistry. April 2020.

S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.

Montana Molecular產(chǎn)品主要包括GPCR檢測、細(xì)胞應(yīng)激和毒性相關(guān)檢測工具，為客戶提供了長時(shí)間內(nèi)觀察候選藥物對細(xì)胞過程影響的能力。艾美捷科技是Montana Molecular的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。