微生物轉氨酶重組蛋白/Recombinant Microbial Transglutaminase英文說明書：

Art. No.：T300

Synonym：EC 2.3.2.13; Protein-glutamine-γ-glutamyltransferase

Background info：AndraconTM is a brand for high quality microbial transglutaminase:

?Recombinantly produced in E. coli

?Ultra-pure and highly active

?Batch to batch consistent quality

?License free

?Produced according to an SOP in an ISO9001:2015 certified environment

AndraconTM (Microbial Transglutaminase, MTG) catalyzes the acyl transfer reaction between γ-carboxyamide groups of peptide-bound glutamine residues and a variety or primary amines.

Therefore, AndraconTM can be used to attach primary-amine coupled functional markers like biotin, fluorescent dyes, click chemistry reagents or cytotoxins to proteins, e.g. antibodies.

Essentially, AndraconTM is used for the production of antibody drug conjugates (ADCs).

This product replaces T001 and T153.

Molecular Weight：38334.0 Da (theoretical, activated MTG with N-terminal sequence: FRAPDSDDR...)

Source：Recombinantly produced in E. coli. Gene derived from Streptomyces mobaraensis.

Specific Activity：> 30 U/mg

Appearance：White lyophilized solid.

Formulation：The purified transglutaminase is lyophilized from 50 mM HEPES pH 7.4.

Reconstitution：Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves.

Application：Labeling, immobilisation, conjugation, and modification of proteins.

Storage：Store at -80°C. Upon reconstitution, store working aliquots undiluted.

If storage at -80°C is not possible, storage at ≤ -20°C is recommended.

艾美捷微生物轉氨酶重組蛋白/Recombinant Microbial Transglutaminase中文說明書：

產品編號：T300

同義詞：EC 2.3.2.13; 蛋白谷氨酰胺γ-谷氨酰轉移酶

背景信息：AndraconTM 是高品質微生物轉谷氨酰胺酶的品牌：

?在大腸桿菌中重組生產

?超純度高活性

?批間質量一致

?無需許可

在ISO9001:2015認證環(huán)境下，按照標準操作程序生產

AndraconTM（微生物轉谷氨酰胺酶，MTG）催化肽結合谷氨酰胺殘基的γ-酰胺基團與各種主要胺之間的酰基轉移反應。因此，AndraconTM 可用于將具有主要胺的功能性標記物（如生物素、熒光染料、點擊化學試劑或細胞毒素）附著到蛋白質上，例如抗體。

本質上，AndraconTM 用于生產抗體藥物偶聯物（ADCs）。

此產品替代了T001和T153。

分子量：38334.0 Da（理論值，激活的MTG具有N末端序列：FRAPDSDDR...）

來源：在大腸桿菌中重組生產。基因源自Streptomyces mobaraensis。

比活性：> 30 U/mg

外觀：白色凍干固體。

配方：純化的轉谷氨酰胺酶從50 mM HEPES pH 7.4中凍干。

復溶：向凍干粉末的小瓶中加入與蛋白質凍干前相同的體積的水（見分析證書）。輕輕旋轉小瓶直到固體溶解。

應用：蛋白質的標記、固定、偶聯和修飾。

儲存：在-80°C下儲存。復溶后，儲存未稀釋的工作量分裝。

如果無法在-80°C下儲存，建議在≤ -20°C下儲存。

Andracon微生物轉氨酶重組蛋白/Recombinant Microbial Transglutaminase參考文獻：

Kaempffe et al., J. Pharm. Sci. 2021, S0022-3549(21)00400-7;

Früh et al., ACS Nano 2021, 15, 12161–70;

Stricker et al., J. Pediatr. Gastroenterol. Nutr. 2019, 68:e43-e50;

Spycher et al., ChemBioChem 2017, 18:1923-7;

Steffen et al., J. Biol. Chem. 2017, 292:15622-35;

Dennler et al., Chembiochem. 2015, 16:861-7;

Dennler et al., Bioconjugate Chem. 2014, 25, 569-78;

Kaufmann et al., Food Addit. Contam. Part. A 2012, 29:1364-73;

Jeger et al., Angew. Chem. Int. Ed. Engl. 2010, 49:9995-7;

Gianfrani et al., Gastroenterology 2007, 133:780-9;

Pfleiderer et al., Microbiol. Res. 2005, 160:265-71;

Ando et al., Agric. Biol. Chem. 1989, 53:2613-17;

Pasternack et al., Eur. J. Biochem. 1998, 257:570-6

僅用于研究用途，不用于人類、治療或診斷應用。