TG2重組蛋白/Recombinant TG2英文說明書：

Art. No.：T122

Synonym：Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase, His6-rhTG2

Molecular Weight：78 kDa

Source：Recombinantly produced in insect cells

Purity：> 95% [SDS-PAGE and Coomassie staining]

The gel shows a single dominant protein band at ~80 kDa.

Appearance：White lyophilized solid

Reagents：The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL Maltodextrin.

Reconstitution：Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.

Application：The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.

Coating：Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.

Storage：T122 is stable for at least two years if stored at ≤ -20°C.

Delivery is possible at ambient temperature

Note：Intended for research use only, not for use in human, therapeutic or diagnostic applications.

艾美捷TG2重組蛋白/Recombinant TG2中文說明書：

產(chǎn)品編號：T122

同義詞：組織型轉(zhuǎn)谷氨酰胺酶，TG2，TGase 2，蛋白谷氨酰胺γ-谷氨酰轉(zhuǎn)移酶，His6-rhTG2

分子量：78 kDa

來源：在昆蟲細(xì)胞中重組生產(chǎn)

純度：

95% [通過SDS-PAGE和Coomassie染色檢測]

凝膠顯示一個主要的蛋白條帶，大約在80 kDa。

外觀：白色凍干固體

試劑：轉(zhuǎn)谷氨酰胺酶是從10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL 麥芽糊精中凍干得到的。

復(fù)溶：向凍干粉末的小瓶中加入與蛋白質(zhì)凍干前相同的體積的水（見分析證書）。輕輕旋轉(zhuǎn)小瓶直到固體溶解。復(fù)溶后，溶液應(yīng)分裝后冷凍儲存。短期儲存時，請保持在冰上冷卻。

應(yīng)用：重組抗原適用于固相（酶聯(lián)免疫吸附試驗(yàn)ELISA和免疫印跡）和液相診斷檢測。該蛋白可被人IgA和IgG（自身）抗體結(jié)合。

包被：用您的包被緩沖液稀釋至適當(dāng)濃度，例如1 μg/ml。請注意，包被條件需要仔細(xì)評估。

儲存：如果儲存在≤ -20°C，T122至少穩(wěn)定兩年。可以在常溫下交付。

注意：僅供研究使用，不適用于人體、治療或診斷應(yīng)用。

僅供研究使用，不適用于人體、治療或診斷應(yīng)用。