DNA甲基化是哺乳動物基因組最常見的修飾方式，它是在DNA甲基轉移酶（DNMTs）的催化下，以甲硫氨酸為供體，將甲基轉移到胞嘧啶的第五個碳原子上。DNA甲基化是一個可逆的過程，在體內同時存在著主動或被動的去甲基化過程。     

       DNA甲基化主要發(fā)生在5'-CpG-3'的C上，在DNA甲基轉移酶的催化下生成5-甲基胞嘧啶(5mC)。DNA甲基化的主要有三種酶:DNMT1，DNMT2，DNMT3a和DNMT3b以及他們的亞型。DNMT1是細胞中最重要的甲基轉移酶，具有從頭和維持甲基化的功能，DNMT1對胚胎的正常發(fā)育至關重要，并與基因組的不穩(wěn)定性有關，DNMT1能與多種蛋白相互作用，參與基因的沉默，在許多腫瘤的發(fā)生和發(fā)展中都觀察到DNMT1的過表達；DNMT2的生物功能到目前為止還不清楚；DNMT3a和DNMT3b是主要的從頭甲基化的酶，許多原發(fā)腫瘤和轉化細胞中都觀察到了DNMT3B的過表達，DNMT3B在腫瘤發(fā)生的過程中對啟動子的過甲基化有很重要的作用；DNMTs之間的作用也非常復雜。     

       近年來，DNA甲基化和人類疾病，尤其是和腫瘤之間關系引起研究人員的關注。許多研究發(fā)現(xiàn)，DNA甲基化模式的改變導致了腫瘤的發(fā)生。在很多腫瘤中都發(fā)現(xiàn)DNMTs的表達異常。這說明DNMTs在生物體內發(fā)揮著重要的作用。     艾美捷科技作為專業(yè)的生命科學領域解決方案供應商，為您推薦全球最暢銷的DNA甲基轉移酶（DNMTs)活性/抑制檢測試劑盒：

       DNA甲基轉移酶活性/抑制試劑盒原理：  

       EpiQuik DNMT（DNA甲基轉移酶）活性/抑制分析超級試劑盒是無放射性實驗.通用的DNMT底物穩(wěn)定的包被在孔板里，甲基轉移酶把甲硫氨酸的甲基集團轉移給胞嘧啶，甲基化DNA底物.甲基化的DNA能被抗-5-甲基化胞嘧啶的抗體所識別.比率或者大量甲基化的DNA與酶的活性成比例，然后類似Elisa的反應，比色法測定DNMT的活性，其吸收波長為450nm，DNMT的活性與光密度值成比例.  

       DNA甲基轉移酶活性/抑制試劑盒試劑盒優(yōu)勢：  【全覆蓋的適用種屬】：哺乳動物（細胞，組織），植物，真菌，細菌等  

       1、快捷的實驗操作，完成實驗不超過3個小時45分鐘；  

       2、安全創(chuàng)新性的比色實驗不需要放射性物質，提取或者親和層析；  

       3、超靈敏檢測極限：核提取物低至0.5ug，純化酶低至0.5ng，靈敏度是前任試劑盒的5倍；  

       4、最優(yōu)化的抗體和增強液能高特異性的檢測5-mc，與未甲基化的胞嘧啶沒有交叉反應；  

       5、微孔板分析，既能適用手工操作，又能適用于高通量分析.

       實驗流程與發(fā)表文章結果展示：

•        產(chǎn)品說明書：

       比色法：https://www.epigentek.com/docs/P-3009.pdf

       熒光法：https://www.epigentek.com/docs/P-3010.pdf     

       《已發(fā)表文章》

 

 

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2. George AK  al.(March 2018). Exercise Mitigates Alcohol Induced Endoplasmic Reticulum Stress Mediated Cognitive Impairment through ATF6-Herp Signaling. Sci Rep. 8(1):5158.

 

 

3. Shahid M  al.(March 2018). Alpha-oxoglutarate inhibits the proliferation of immortalized normal bladder epithelial cells via an epigenetic switch involving ARID1A. Sci Rep. 8(1):4505.

 

 

4. Søvik E  al.(February 2018). Cocaine Directly Impairs Memory Extinction and Alters Brain DNA Methylation Dynamics in Honey Bees Front. Physiol.. 9

 

 

5. Li W  al.(December 2017). Curcumin Derivative Epigenetically Reactivates Nrf2 Antioxidative Stress Signaling in Mouse Prostate Cancer TRAMP C1 Cells. Chem Res Toxicol.

 

 

6. Kuc C  al.(December 2017). Rainbow trout exposed to benzo[a]pyrene yields conserved microRNA binding sites in DNA methyltransferases across 500 million years of evolution Scientific Reports. 7

 

 

7. Nanduri J  al.(November 2017). DNA methylation in the central and efferent limbs of the chemo reflex requires carotid body neural activity. J Physiol.

 

 

8. Carden T  al.(October 2017). Epigenetic modification of miR-663 controls mitochondria-to-nucleus retrograde signaling and tumor progression. J Biol Chem.

 

 

9. Fu S  al.(September 2017). 5-Aza-2'-deoxycytidine induces human Tenon's capsule fibroblasts differentiation and fibrosis by up-regulating TGF-β type I receptor. Exp Eye Res.

 

 

10. Wirbisky-Hershberger SE  al.(August 2017). Atrazine exposure decreases the activity of DNMTs, global DNA methylation levels, and dnmt expression. Food Chem Toxicol.

 

 

11. Li Y  al.(August 2017). Combinatorial bioactive botanicals re-sensitize tamoxifen treatment in ER-negative breast cancer via epigenetic reactivation of ERα expression. Sci Rep. 7(1):9345.

 

 

12. Li  al.(August 2017). Effects of dietary l-methionine supplementation on the growth performance, carcass characteristics, meat quality, and muscular antioxidant capacity and myogenic gene expression in low birth weight pigs J. Anim. Sci..

 

 

13. Xu Y  al.(July 2017). Intracellular adenosine regulates epigenetic programming in endothelial cells to promote angiogenesis.EMBO Mol Med.

 

 

14. Xu H  al.(May 2017). DNMT1 regulates IL-6- and TGF-β1-induced epithelial mesenchymal transition in prostate epithelial cells. Eur J Histochem. 61(2):2775.

 

 

15. Yin J  al.(April 2017). Acylamino acid chiral fungicides on toxiciepigenetics in lambda DNA methylation. Food Chem Toxicol.

 

 

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17. Fan Yang  al.(December 2016). Casticin inhibits the activity of transcription factor Sp1 and the methylation of RECK in MGC803 gastric cancer cells Experimental and Therapeutic Medicine. 13(2):745-750.

 

 

18. Sanchez OF  al.(December 2016). Lead (Pb) exposure reduces global DNA methylation level by non-competitive inhibition and alteration of dnmt expression. Metallomics.

 

 

19. Vibe Nylander  al.(September 2016). Ionizing Radiation Potentiates High Fat Diet-Induced Insulin Resistance and Reprograms Skeletal Muscle and Adipose Progenitor Cells ADA. 65(10)

 

 

20. Herencia C  al.(June 2016). Procaine Inhibits Osteo/Odontogenesis through Wnt/β-Catenin Inactivation. PLoS One.11(6):e0156788.

 

 

21. Wijenayake S  al.(February 2016). The role of DNA methylation during anoxia tolerance in a freshwater turtle (Trachemys scripta elegans). J Comp Physiol B.

 

 

22. Poh WJ  al.(January 2016). DNA Methyltransferase Activity Assays: Advances and Challenges Theranostics. 6(3):369-391.

 

 

23. Sun R  al.(November 2015). Deoxyribonucleic acid (DNA) methyltransferase contributes to p16 promoter CpG island methylation in lung adenocarcinoma with smoking Int J Clin Exp Med. 8(9):15773-15779.

 

 

24. Naselli F  al.(October 2015). Phytochemical Indicaxanthin Inhibits Colon Cancer Cell Growth and Affects the DNA Methylation Status by Influencing Epigenetically Modifying Enzyme Expression and Activity. J Nutrigenet Nutrigenomics. 8(3):114-27.

 

 

25. Pushpakumar S  al.(July 2015). DNA hypermethylation in hyperhomocysteinemia contributes to abnormal extracellular matrix metabolism in the kidney. FASEB J.

 

 

26. Li JJ  al.(June 2015). Homocysteine Triggers Inflammatory Responses in Macrophages through Inhibiting CSE-H2S Signaling via DNA Hypermethylation of CSE Promoter. Int J Mol Sci. 16(6):12560-12577.

 

 

27. Pathania R  al.(April 2015). DNMT1 is essential for mammary and cancer stem cell maintenance and tumorigenesis. Nat Commun. 6:6910.

 

 

28. Zeng F  al.(March 2015). Dynamic change of DNA methylation and cell redox state at different micropropagation phases in birch. Trees. :1-14.

 

 

29. Ishikawa K  al.(February 2015). Long-term pancreatic beta cell exposure to high levels of glucose but not palmitate induces DNA methylation within the insulin gene promoter and represses transcriptional activity. PLoS One. 10(2):e0115350.

 

 

30. Bian Y  al.(February 2015). Epigenetic Regulation of miR-29s Affects the Lactation Activity of Dairy Cow Mammary Epithelial Cells. J Cell Physiol.

 

 

31. Cho SH  al.(January 2015). SIRT1 deficiency in microglia contributes to cognitive decline in aging and neurodegeneration via epigenetic regulation of IL-1β. J Neurosci. 35(2):807-18.

 

 

32. Cheng C  al.(December 2014). 'Default' generated neonatal regulatory T cells are hypomethylated at conserved non-coding sequence 2 and promote long-term cardiac allograft survival. Immunology. 143(4):618-30.

 

 

33. Zhang S  al.(December 2014). The Effect of Hypoxia Preconditioning on DNA Methyltransferase and PP1γ in Hippocampus of Hypoxia Preconditioned Mice. High Alt Med Biol. 15(4):483-90.

 

 

34. Cardenas H  al.(November 2014). TGF-β induces global changes in DNA methylation during the epithelial-to-mesenchymal transition in ovarian cancer cells. Epigenetics.

 

 

35. Chen S  al.(November 2014). Identifying Novel Selective Non-Nucleoside DNA Methyltransferase 1 Inhibitors through Docking-Based Virtual Screening. J Med Chem. 57(21):9028-41.

 

 

36. Choi YW  al.(November 2014). B-RafV600E inhibits sodium iodide symporter expression via regulation of DNA methyltransferase 1. Exp Mol Med. 46:e120.

 

 

37. Wang L  al.(November 2014). Blocking of JB6 Cell Transformation by Tanshinone IIA: Epigenetic Reactivation of Nrf2 Antioxidative Stress Pathway. AAPS J. 16(6):1214-25.

 

 

38. Pudenz M  al.(October 2014). Impact of soy isoflavones on the epigenome in cancer prevention. Nutrients. 6(10):4218-72.

 

 

39. Zhu Y  al.(September 2014). Placental mesenchymal stem cells of fetal origin deposit epigenetic alterations during long-term culture under serum-free condition. Expert Opin Biol Ther. :1-18.

 

 

40. Lai J  al.(August 2014). Transcriptional regulation of the p73 gene by Nrf-2 and promoter CpG methylation in human breast cancer. Oncotarget. 5(16):6909-22.

 

 

41. Wang J  al.(July 2014). MicroRNA-152 Regulates DNA Methyltransferase 1 and Is Involved in the Development and Lactation of Mammary Glands in Dairy Cows. PLoS One. 9(7):e101358.

 

 

42. Sakharkar AJ  al.(June 2014). Effects of acute ethanol exposure on anxiety measures and epigenetic modifiers in the extended amygdala of adolescent rats. Int J Neuropsychopharmacol. :1-11.

 

 

43. Scola G  al.(April 2014). Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons. Psychopharmacology (Berl).

 

 

44. Park J  al.(April 2014). Hepatitis C virus represses E-cadherin expression via DNA methylation to induce epithelial to mesenchymal transition in human hepatocytes. Biochem Biophys Res Commun. 446(2):561-7.

 

 

45. Chaturvedi P  al.(April 2014). Differential regulation of DNA methylation versus histone acetylation in cardiomyocytes during HHcy in vitro and in vivo: an epigenetic mechanism. Physiol Genomics. 46(7):245-55.

 

 

46. Lin N  al.(April 2014). Homocysteine induces cytotoxicity and proliferation inhibition in neural stem cells via DNA methylation in vitro. FEBS J. 281(8):2088-96.

 

 

47. Wu J  al.(March 2014). Kaposi's sarcoma-associated herpesvirus (KSHV) vIL-6 promotes cell proliferation and migration by upregulating DNMT1 via STAT3 activation. PLoS One. 9(3):e93478.

 

 

48. Wang Y  al.(March 2014). Ras-induced epigenetic inactivation of Ras-related associated with diabetes gene (RRAD) promotes glucose uptake in a human ovarian cancer model. J Biol Chem.

 

 

49. Zhang X  al.(February 2014). Regulation of DNA methylation by ethanol induces tissue plasminogen activator expression in astrocytes. J Neurochem. 128(3):344-9.

 

 

50. Muren NB  al.(November 2013). Electrochemical assay for the signal-on detection of human DNA methyltransferase activity. J Am Chem Soc. 135(44):16632-40.

 

 

51. Liao W  al.(November 2013). Enhancing bone marrow regeneration by SALL4 protein. J Hematol Oncol. 6:84.

 

 

52. Lee H  al.(August 2013). DNA methyltransferase inhibitor assay system based on the HBx-induced DNA methylation of E-cadherin. Biochem Biophys Res Commun. 438(3):540-5.

 

 

53. Williams-Karnesky RL  al.(August 2013). Epigenetic changes induced by adenosine augmentation therapy prevent epileptogenesis. J Clin Invest. 123(8):3552-63.

 

 

54. Chen Z  al.(July 2013). Epigenetic regulation of cardiac progenitor cells marker c-kit by stromal cell derived factor-1α. PLoS One. 8(7):e69134.

 

 

55. Yara S  al.(May 2013). Total parenteral nutrition induces sustained hypomethylation of DNA in newborn guinea pigs. Pediatr Res. 73(5):592-5.

 

 

56. Kauntz H  al.(April 2013). Epigenetic effects of the natural flavonolignan silibinin on colon adenocarcinoma cells and their derived metastatic cells. Oncol Lett. 5(4):1273-1277.

 

 

57. Gao F  al.(October 2012). Differential DNA methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis. Genome Biol. 13(10):R100.

 

 

58. Tewari S  al.(July 2012). Mitochondria DNA replication and DNA methylation in the metabolic memory associated with continued progression of diabetic retinopathy. Invest Ophthalmol Vis Sci. 53(8):4881-8.

 

 

59. Yang J  al.(January 2012). Stem cell gene SALL4 suppresses transcription through recruitment of DNA methyltransferases. J Biol Chem. 287(3):1996-2005.

 

 

60. Yiming Xu  al.(July 2017). Intracellular adenosine regulates epigenetic programming in endothelial cells to promote angiogenesis EMBOpress Molecular Medicine.

 

 

61. Wu Z  al.(June 2017). Ultraviolet B inhibition of DNMT1 activity via AhR activation dependent SIRT1 suppression in CD4+ T cells from systemic lupus erythematosus patients. J Dermatol Sci. 86(3):230-237.

 

 

62. Nugent BM  al.(May 2015). Brain feminization requires active repression of masculinization via DNA methylation. Nat Neurosci. 18(5):690-7.

 

 

63. Wang B  al.(January 2015). Exposure to low dose cadmium enhances FL83B cells proliferation through down-regulation of caspase-8 by DNA hypermethylation Toxicol. Res.. (Advance Article) Abstract

 

 

64. Olsvik PA  al.(July 2014). Impact of dietary selenium on methylmercury toxicity in juvenile Atlantic cod: A transcriptional survey. Chemosphere. 120C:199-205.

 

 

65. Dunn J  al.(May 2014). Flow-dependent epigenetic DNA methylation regulates endothelial gene expression and atherosclerosis. J Clin Invest.

 

 

66. Zhang D  al.(October 2012). Homocysteine activates vascular smooth muscle cells by DNA demethylation of platelet-derived growth factor in endothelial cells. J Mol Cell Cardiol. 53(4):487-96.

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