【前情回顧】DNA甲基化（5-mc）極速分析試劑盒     DNA甲基化免疫共沉淀技術(shù)(MeDIP or mDIP) ，是一個大范圍的染色體或基因組純化技術(shù)，在分子生物學中被用于富集DNA甲基化序列，它采用抗體或甲基化DNA結(jié)合蛋白來捕獲富集甲基化DNA的技術(shù)，這種技術(shù)可以發(fā)現(xiàn)基因組中高度甲基化的區(qū)域，如CpG島。MeDIP技術(shù)特別適用于高通量測序以及芯片雜交檢測。

《MeDIP 的兩種廣泛應(yīng)用》

      艾美捷科技作為專業(yè)的生命科學領(lǐng)域解決方案供應(yīng)商，為您推薦來源于全球最暢銷，發(fā)表文章最多的甲基化DNA免疫沉淀（MeDIP）試劑盒：   

•       該試劑盒需要的樣品：提取好的基因組DNA

       MeDIP試劑盒原理：  Methylamp甲基化DNA免疫沉淀試劑盒中含有捕獲樣品中甲基化DNA所需的所有試劑，并且，本試劑盒中還有一種CHIP-級5-MC抗體，以及正常小鼠的IgG陰性對照.DNA打斷之后，加入微孔板中，被抗體捕獲.然后DNA從復合物上解離下來，通過本公司特別設(shè)計的高速離心柱子純化，洗脫下來的DNA可以被用于隨后的各種擴增反應(yīng)。

        MeDIP試劑盒優(yōu)勢：

      1、高效地濃縮富集甲基化DNA，>98%；

      2、同類產(chǎn)品中最快速的試劑盒，3小時內(nèi)完成反應(yīng)；

      3、96孔板模式使研究人員能根據(jù)自己需要選擇手工或是高通量分析；

      4、附有DNA純化柱子：節(jié)省時間和人力；

      5、操作簡便、結(jié)果可靠、統(tǒng)一的分析條件；

      實驗流程示意圖與結(jié)果展示：  

•       產(chǎn)品說明書：https://www.epigentek.com/docs/P-1015.pdf

   《已發(fā)表28篇文章》

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      van der Wijst MG et. al. (December 2017). Experimental mitochondria-targeted DNA methylation identifies GpC methylation, not CpG methylation, as potential regulator of mitochondrial gene expression. Sci Rep. 7(1):177.

      Daraei A et. al. (July 2017). Epigenetic Changes of the ESR1 Gene in Breast Tissue of Healthy Women: A Missing Link with Breast Cancer Risk Factors? Genet Test Mol Biomarkers.  

      Wang HD et. al. (June 2017). Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis. Mol Med Rep. 15(6):3989-3998.

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      Manish Mishra; Renu A. Kowluru et. al. (October 2016). The Role of DNA Methylation in the Metabolic Memory Phenomenon Associated With the Continued Progression of Diabetic Retinopathy. IOVS. 57:5748-5757.

      Xu X et. al. (March 2016). Hypoxia-induced Endothelial-Mesenchymal Transition is associated with RASAL1 promoter hypermethylation in human coronary endothelial cells. FEBS Lett.

      Li W et. al. (March 2016). Epigenetics reactivation of Nrf2 in Prostate TRAMP C1 Cells by curcumin analog FN1. Chem Res Toxicol.

       Tan X et. al. (January 2016). DNMT1 and HDAC2 Cooperate to Facilitate Aberrant Promoter Methylation in Inorganic Phosphate-Induced Endothelial-Mesenchymal Transition. PLoS One. 11(1):e0147816.

      Tan X et. al. (January 2016). High inorganic phosphate causes DNMT1 phosphorylation and subsequent fibrotic fibroblast activation. Biochem Biophys Res Commun.

      Mishra M et. al. (August 2015). Epigenetic Modification of Mitochondrial DNA in the Development of Diabetic Retinopathy. Invest Ophthalmol Vis Sci. 56(9):5133-42.

      Guo Y et. al. (March 2015). Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1. Biochem Pharmacol. 94(2):69-78.  

      Hong S et. al. (January 2015). Epigenetic regulation of genes that modulate chronic stress-induced visceral pain in the peripheral nervous system. Gastroenterology. 148(1):148-157.e7.

      Tampe B et. al. (December 2014). Induction of Tet3-dependent Epigenetic Remodeling by Low-dose Hy. EBioMedicine. 1(1)

      Green TJ et. al. (August 2014). Anti-viral gene induction is absent upon secondary challenge with double-stranded RNA in the Pacific oyster, Crassostrea gigas. Fish Shellfish Immunol. 39(2):492-7.

      Skowronki K et. al. (July 2014). Genome-Wide Analysis in Human Colorectal Cancer Cells Reveals Ischemia-Mediated Expression of Motility Genes via DNA Hypomethylation. PLoS One. 9(7):e103243.

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      Pol Bodetto S et. al. (October 2013). Cocaine represses protein phosphatase-1Cβ through DNA methylation and Methyl-CpG Binding Protein-2 recruitment in adult rat brain. Neuropharmacology. 73:31-40.

      Zhang TY et. al. (January 2013). Epigenetic mechanisms for the early environmental regulation of hippocampal glucocorticoid receptor gene expression in rodents and humans. Neuropsychopharmacology. 38(1):111-23.  

      Tricker PJ et. al. (June 2012). Low relative humidity triggers RNA-directed de novo DNA methylation and suppression of genes controlling stomatal development. J Exp Bot. 63(10):3799-813.

      Yu F et. al. (January 2012). MicroRNA 34c gene down-regulation via DNA methylation promotes self-renewal and epithelial-mesenchymal transition in breast tumor-initiating cells. J Biol Chem. 287(1):465-73.

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      Hicks SD et. al. (September 2010). Ethanol-induced methylation of cell cycle genes in neural stem cells. J Neurochem.114(6):1767-80.  

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