前情提示：上一期我們給廣大研究者朋友介紹了全球暢銷的DNMT酶活性分析試劑盒，可回顧：http://www.bio-review.com/dnmts-kit/

        DNA甲基化的主要有三種酶:DNMT1，DNMT2，DNMT3a和DNMT3b以及他們的亞型。DNMT3a和DNMT3b是主要的從頭甲基化的酶，許多原發(fā)腫瘤和轉(zhuǎn)化細胞中都觀察到了DNMT3B的過表達，DNMT3B在腫瘤發(fā)生的過程中對啟動子的過甲基化有很重要的作用；DNMTs之間的作用也非常復(fù)雜。許多研究發(fā)現(xiàn)，DNA甲基化模式的改變導(dǎo)致了腫瘤的發(fā)生。在很多腫瘤中都發(fā)現(xiàn)DNMTs的表達異常。這說明DNMTs在生物體內(nèi)發(fā)揮著重要的作用。

DNMTs與癌癥的關(guān)聯(lián)

       艾美捷科技作為專業(yè)的生命科學(xué)領(lǐng)域解決方案供應(yīng)商，為您推薦全球最暢銷的DNMT1, DNMT3A, DNMT3B定量試劑盒：

       *每個試劑盒均被大量文獻引用，見頁面底部

       DNMT酶活性分析試劑盒原理【DNMT1為例】：

       EpiQuik的DNA甲基轉(zhuǎn)移酶1檢測試劑盒專為定量檢測組織或細胞中的總Dnmt1而設(shè)計。在檢測實驗中，微孔上包被有本公司特有的Dnmt吸附基質(zhì)。上樣后，樣品中的Dnmt1結(jié)合到基質(zhì)上。被結(jié)合的Dnmt1能被特異性的Dnmt1抗體識別，可以通過類酶聯(lián)免疫吸附反應(yīng)后測定其比色度來定量，Dnmt1的量與顯色程度成線性關(guān)系。

       DNMT酶活性分析試劑盒優(yōu)勢：

       1、操作十分快捷，3小時內(nèi)可完成

       2、安全獨創(chuàng)的比色度檢測方法，不接觸放射性物質(zhì)，無需進行抽提和色譜分析

       3、96孔板模式使研究人員能根據(jù)自己需要選擇手工或是高通量分析

       4、操作簡便、結(jié)果可靠、統(tǒng)一的分析條件

       5、適用于人與小鼠樣品

《DNMT1試劑盒已發(fā)表文章》

       Swathy B et. al. (January 2018). Understanding the influence of antipsychotic drugs on global methylation events and its relevance in treatment response. Epigenomics.

       Li YC et. al. (September 2017). Procaine is a specific DNA methylation inhibitor with anti-tumor effect for human gastric cancer.J Cell Biochem.

       Rajpathak SN et. al. (February 2017). Micro RNAs and DNA methylation are regulatory players in human cells with altered X chromosome to autosome balance. Sci Rep. 7:43235.

       Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.

       Wu F et. al. (March 2016). Oral administration of Schisandra chinensis extract suppresses Dnmt1 expression in Kunming mice ovaries Genes Genom. :1-8.

       Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.

       Mukhopadhyay P et. al. (October 2015). Cigarette smoke induces proteasomal-mediated degradation of DNA methyltransferases and methyl CpG-/CpG domain-binding proteins in embryonic orofacial cells. Reprod Toxicol.

       Yang G et. al. (July 2015). DNA methyltransferase 3, a target of microRNA-29c, contributes to neuronal proliferation by regulating the expression of brain-derived neurotrophic factor. Mol Med Rep. 12(1):1435-42.

       Selokar NL et. al. (April 2015). Downregulation of DNA Methyltransferase 1 in Zona-Free Cloned Buffalo (Bubalus bubalis) Embryos by Small Interefering RNA Improves In Vitro Development But Does Not Alter DNA Methylation Level. Cell Reprogram.17(2):89-94.

       Das DS et. al. (December 2014). Anti-Myeloma Activity of a Novel Free Radical Inducer Rrx-001 Blood. 124(21):4712-4712.

       Chu M et. al. (April 2014). Hypermethylation-mediated transcriptional repression of TMPRSS2 in androgen receptor-negative prostate cancer cells. Exp Biol Med (Maywood).

       Bielak-Zmijewska A et. al. (February 2014). A comparison of replicative senescence and doxorubicin-induced premature senescence of vascular smooth muscle cells isolated from human aorta. Biogerontology. 15(1):47-64.

       Nettersheim D et. al. (December 2013). Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development. PLoS One. 8(12):e82881.

       Wu Z et. al. (September 2013). Ultraviolet B enhances DNA hypomethylation of CD4+ T cells in systemic lupus erythematosus via inhibiting DNMT1 catalytic activity. J Dermatol Sci. 71(3):167-73.

       Jang JY et. al. (February 2013). ANT2 suppression by shRNA may be able to exert anticancer effects in HCC further by restoring SOCS1 expression. Int J Oncol. 42(2):574-82.

       Zhao J et. al. (January 2013). Genome-wide identification of Epstein-Barr virus-driven promoter methylation profiles of human genes in gastric cancer cells. Cancer. 119(2):304-12.

       Desplats P et. al. (March 2011). Alpha-synuclein sequesters Dnmt1 from the nucleus: a novel mechanism for epigenetic alterations in Lewy body diseases. J Biol Chem. 286(11):9031-7.

       Jang JY et. al. (September 2010). Short-hairpin RNA-induced suppression of adenine nucleotide translocase-2 in breast cancer cells restores their susceptibility to TRAIL-induced apoptosis by activating JNK and modulating TRAIL receptor expression.Mol Cancer. 9:262.

       Hervouet E et. al. (June 2010). Disruption of Dnmt1/PCNA/UHRF1 interactions promotes tumorigenesis from human and mice glial cells. PLoS One. 5(6):e11333.

       Liu Z et. al. (May 2009). Modulation of DNA methylation by a sesquiterpene lactone parthenolide. J Pharmacol Exp Ther.329(2):505-14.

       Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.

       Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.

1. D. Roll et. al.(January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines Molecular Cancer.7(15) Full PDF Article

 

 

《DNMT3A試劑盒已發(fā)表文章》

       Swathy B et. al. (January 2018). Understanding the influence of antipsychotic drugs on global methylation events and its relevance in treatment response. Epigenomics.

       Coco M et. al. (November 2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise.Medicine (Baltimore). 96(44):e8325.

       Parira T et. al. (September 2017). Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci Rep. 7(1):11236.

       Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.

       Horsburgh S et. al. (December 2015). Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiol Rep. 3(12)  Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.

       Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.

《DNMT3B試劑盒已發(fā)表文章》

       Coco M et. al. (November 2017). Effects of age and sex on epigenetic modification induced by an acute physical exercise.Medicine (Baltimore). 96(44):e8325.

       Cechinel LR et. al. (October 2016). Treadmill exercise induces age and protocol-dependent epigenetic changes in prefrontal cortex of Wistar rats. Behav Brain Res. 313:82-7.

       Mytych J et. al. (February 2016). Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells. Mol Neurobiol.

       Horsburgh S et. al. (December 2015). Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells. Physiol Rep. 3(12)

       Zhao J et. al. (January 2013). Genome-wide identification of Epstein-Barr virus-driven promoter methylation profiles of human genes in gastric cancer cells. Cancer. 119(2):304-12.

       Jin Y et. al. (June 2011). Homocysteine levels impact directly on epigenetic reprogramming in astrocytes. Neurochem Int.58(7):833-8.

       Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.

       Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.

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