His標(biāo)簽，也稱為多組氨酸標(biāo)簽（polyhistidine），His6標(biāo)簽或hexa組氨酸標(biāo)簽，這是一種在轉(zhuǎn)染的細(xì)胞中目標(biāo)蛋白的C端或N端上由至少6個組氨酸殘基鏈接上所組成的氨基酸序列。

His-Tag是目前用于純化的融合標(biāo)簽中應(yīng)用最廣泛的特點(diǎn)：

a：標(biāo)簽較短可置于蛋白的N端或者C端

b：對蛋白的功能及下游應(yīng)用影響小

c：免疫原性低，可以和多種標(biāo)簽連用

d：純化方式非常簡單方便

這些屬性使His標(biāo)簽成為重組蛋白生產(chǎn)和分離的流行標(biāo)簽。然而，對于未純化的樣本，His標(biāo)簽蛋白的準(zhǔn)確定量樣品仍然很困難。

作為專業(yè)的生命科學(xué)醫(yī)藥原料供應(yīng)商，Cell Biolabs中國區(qū)金牌代理，艾美捷科技為您推薦：His-Tag 蛋白ELISA 試劑盒，這是一種基于競爭性ELISA測定法，專為快速檢測和定量His標(biāo)簽蛋白樣品（C 端或 N 端）而開發(fā)。

引用文獻(xiàn)：

1.Helalat, S.H. et al. (2020). Investigating the efficacy of UVSE protein at repairing CPD and 6–4 pp DNA damages in human cells. J Photochem Photobiol B. doi: 10.1016/j.jphotobiol.2020.111843.

2.Huang, Y. et al. (2020). Dual-mechanism based CTLs infiltration enhancement initiated by Nanosapper potentiates immunotherapy against immune-excluded tumors. Nat Commun. 11(1):622. doi: 10.1038/s41467-020-14425-7.

3.Karamitros, C.S. et al. (2020). Bacterial expression systems for enzymatic activity in droplet-based microfluidics. Anal Chem. doi: 10.1021/acs.analchem.9b04969.

4.Arnett, C. et al. (2019). Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Appl Microbiol Biotechnol. doi: 10.1007/s00253-019-10141-5.

5.Liu, Q. et al. (2018). Nanoparticle-Mediated Trapping of Wnt Family Member 5A in Tumor Microenvironments Enhances Immunotherapy for B-Raf Proto-Oncogene Mutant Melanoma. ACS Nano. 12(2):1250-1261. doi: 10.1021/acsnano.7b07384.

6.Ibarra-Sánchez, L.A. et al. (2018). Antimicrobial behavior of phage endolysin PlyP100 and its synergy with nisin to control Listeria monocytogenes in Queso Fresco. Food Microbiol. 72:128- 134. doi: 10.1016/j.fm.2017.11.013.

7.Reyes-Corona, D. et al. (2017). Neurturin overexpression in dopaminergic neurons induces presynaptic and postsynaptic structural changes in rats with chronic 6-hydroxydopamine lesion. PLoS One. 12(11):e0188239. doi: 10.1371/journal.pone.0188239.

8.Warnock, N.D. et al. (2017). Nematode neuropeptides as transgenic nematicides. PLoS Pathog. 13(2):e1006237. doi: 10.1371/journal.ppat.1006237.

9.Rinaldo, A. R. et al. (2015). A grapevine anthocyanin acyltransferase, transcriptionally regulated by VvMYBA, can produce most acylated anthocyanins present in grape skins. Plant Physiol. 169:1897-916.

10.Akiyama, Y. et al. (2014). The identification of affinity peptide ligands specific to the variable region of human antibodies. Biomed Res. 35:105-116.

11.Dong, Y. et al. (2013). HMGB1 protein does not mediate the inflammatory response in spontaneous spinal cord regeneration: a hint for CNS regeneration. J. Biol. Chem. 288:18204- 182128.

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