worthington 其他組織分離指南

發(fā)布者：艾美捷科技發(fā)布時(shí)間：2022-06-07

分享到：

原代細(xì)胞分離中，針對其他組織涉及到髓核，肌腱細(xì)胞、牙髓干細(xì)胞、口腔上皮等等的解離通常會(huì)使用酶，如何選擇合適的酶來完成對應(yīng)其他組織的解離，以及原代細(xì)胞采集的常規(guī)操作， 艾美捷針對其他相關(guān)的組織分類解離分種屬、不同的細(xì)胞、酶組合推薦，推薦濃度、培養(yǎng)基以及參考文獻(xiàn)，詳細(xì)匯總指南：其它組織分離指南

本技術(shù)指南描述了標(biāo)準(zhǔn)實(shí)驗(yàn)室程序；為建立細(xì)胞分離方案提供了一種合乎邏輯的實(shí)驗(yàn)方法；并列出了許多組織特異性參考資料，以幫助開發(fā)有效的方法。更多其他組織的解離指南，點(diǎn)擊查看：各組織分離指南。

其他組織
種屬	種屬詳情	細(xì)胞名稱	酶組合推薦，含推薦濃度	培養(yǎng)基	參考文獻(xiàn)
牛	Bovine	髓核 / Nucleus pulposus	Collagenase Type 2: 0.2%	DMEM	Lee, J., Cheung, K. and Leung, V.: Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability., J Orthop Res 33, 1743-55, 2015
馬	Equine	肌腱細(xì)胞 / Tendon Cells	Collagenase Type 1: 0.1%	DMEM	Nemoto, M., Kizaki, K., Yamamoto, Y., Oonuma, T. and Hashizume, K.: Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration., J Equine Sci 24, 17-24, 2013
山羊	Goat	髕下脂肪墊間充質(zhì)基質(zhì)細(xì)胞 / Infrapatellar fat pad mesenchymal stromal	Collagenase: 0.15%	DMEM	Arora, A., Sriram, M., Kothari, A. and Katti, D.: Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering., Cytotherapy 19, 881-894, 2017
人	Human	粘附成纖維細(xì)胞 / Adhesion fibroblasts	Collagenase Type 4: 0.2%	Medium 199	Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
人	Human	髓核 / Nucleus pulposus	Pronase: 0.25%； Collagenase Type 2: 600 u/ml	DMEM	Sun, Y., Lv, M., Zhou, L., Tam, V., Lv, F., Chan, D., Wang, H., Zheng, Z., Cheung, K. and Leung, V.: Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation., Osteoarthritis Cartilage 23, 1194-203, 2015
人	Human, 3-7 year	牙髓干細(xì)胞 / Dental pulp stem cells	Collagenase Type 1: 0.3%； Neutral Protease: 0.4%	DMEM	Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE 8, e56749, 2013
人	Human	牙髓干細(xì)胞 / Dental pulp stem	Collagenase Type 1: 0.3%； Neutral Protease: 0.4%	DMEM/F12	Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol 4, 357, 2013
人	Human	間充質(zhì)基質(zhì)細(xì)胞 / Mesenchymal stromal	Collagenase Type 1: 0.075%	PBS	Lee, Y., Lee, J., Park, H., Lim, Y., Lee, J., Wang, S. and Lee, B.: Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue., Cell Physiol Biochem 31, 513-24, 2013
人	Human	菌狀味乳頭 / Fungiform taste papillae	Collagenase Type 1: 550 u/ml； Elastase: 10 u/ml； Soybean Trypsin Inhibitor: 0.09%	see reference	Ozdener, H., Spielman, A. and Rawson, N.: Isolation and Culture of Human Fungiform Taste Papillae Cells, J Vis Exp 63, e3730, 2012
人	Human	肌腱細(xì)胞 / Tendon cells	Collagenase Type 2: 0,3%	DMEM/Hams F12	Pauly, S., Klatte, F., Strobel, C., Schmidmaier, G., Greiner, S. and Scheibel, M. Wildemann B: Characterization of Tendon Cell Cultures of the Human Rotator Cuff., Eur Cell Mater 20, 84-97, 2010
人	Human	脂肪來源的基質(zhì)細(xì)胞 / Adipose derived stromal	Collagenase Type 2: 0.075%	DMEM	Panetta, N., Gupta, D., Kwan, M., Wan, D., Commons, G. and Longaker, M.: Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells., Plast Reconstr Surg 124, 65, 2009
人	Human	口腔上皮 / Oral epithelial	Neutral Protease: 1.2 u/ml； Trypsin: 0.05%	PBS	Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25, 628-38, 2007
人	Human	頸動(dòng)脈斑塊巨噬細(xì)胞 / Carotid artery plaque macrophage	Collagenase Type 4: 450 u/ml； Deoxyribonuclease I: 500 u/ml； Soybean Trypsin Inhibitor: 0.1%	HBSS	Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM: Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin, Circ Res 98, 1282-9, 2006
人	Human	食管微血管內(nèi)皮 / Esophageal microvascular endothelial	Collagenase Type 2: 0.2%	MCDB-131	Rafiee, P., Ogawa, H., Heidemann, J., Li, M., Aslam, M., Lamirand, T., Fisher, P., Graewin, S., Dwinell, M., Johnson, C., Shaker, R. and Binion, D.: Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation., Am J Physiol Gastrointest Liver Physiol 285, G1277, 2003
人		胃上皮細(xì)胞 / Gastric epithelial cells	Collagenase Type 2: 200 u/ml； Neutral Protease: 1.2 u/ml； Soybean Trypsin Inhibitor: 0.125%	L-15	Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22, 133, 2000
人	Human	滑膜細(xì)胞 / Synoviocytes	Collagenase: 0.2%	DMEM/F12	Chen V, Croft D, Purkis P, Kramer IM: Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release., Br J Rheumatol 37, 148-56, 1998
人	Human	消化細(xì)胞 / Peptic cells	Collagenase Type 4: 0.1%； Soybean Trypsin Inhibitor: 0.2%	Ringer solution	Lanas. A., Nerin, J., Esteva, F. and Sainz, R.: Non-Steroidal Anti-Inflammatory Drugs and Prostaglandin Effects on Pepsinogen Secretion by Dispersed Human Peptic Cells., Gut 36, 657-63, 1995
人	Human	根尖肉芽腫 / Periapical granuloma	CLSPA: 0.25%	RPMI-1640	Stern MH, Dreizen S, Mackler BF, Levy BM: Isolation and characterization of inflammatory cells from the human periapical granuloma, J Dent Res 61, 1408-12, 1982
昆蟲	Insect, Lepidoptera	鱗翅目 / Lepitopteran	Collagenase Type 3: 0.35%； Hyaluronidase: 0.01%	Dulbecco PBS	Goodwin, R and McCawley, P: Initiating Attached Cell Lines From the Lepidoptera (Insecta), Meth Cell Sci 3, 567, 1977
無脊椎動(dòng)物	Squid	地幔 / Mantle	Collagenase: see reference； Papain: see reference	Phosphate buffer	Raman, M. and Mathew, S.: Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny., J Food Sci Technol 51, 1509-16, 2014
小鼠	Mouse	類器官培養(yǎng)物 / Organoid cultures	Stemxyme 1: 0.1%	PBS	Huang, L., Desai, R., Conrad, D., Leite, N., Akshinthala, D., Lim, C., Gonzalez, R., Muthuswamy, L., Gartner, Z. and Muthuswamy, S.: Commitment and Oncogene-induced Plasticity of Human Stem Cell-derived Pancreatic Acinar and Ductal Organoids.,Cell Stem Cell Vol. 28,, , 1090-1104.e6, 2021
小鼠	Mouse, embryonic	氣管 / Trachea	Elastase: 0.1%； Neutral Protease: 0.25%； Deoxyribonuclease I: 0.01%； Trypsin: 0.125%	DMEM/F12	He, M., Wu, B., Ye, W., Le, D., Sinclair, A., Padovano, V., Chen, Y., Li, K., Sit, R., Tan, M., Caplan, M., Neff, N., Jan, Y., Darmanis, S. and Jan, L.: Chloride Channels Regulate Differentiation and Barrier Functions of the Mammalian Airway., Elife 9, 53085, 2020
小鼠	Mouse	粘附成纖維細(xì)胞 / Adhesion fibroblasts	Collagenase Type 4: 0.2%	Medium 199	Foster, D., Marshall, C., Gulati, G., Chinta, M., Nguyen, A., Salhotra, A., Jones, R., Burcham, A., Lerbs, T., Cui, L., King, M., Titan, A., Ransom, R., Manjunath, A., Hu, M. and Blackshear, C.: Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation., Nat Commun 11, 4061, 2020
小鼠	Mouse, 8-12 week	膀胱 / Urinary bladder	Collagenase Type 6: 20 u/mlCollagenase Type 1: 50 u/ml； Collagenase Type 3: 40 u/ml； Papain: 45 u/ml； Deoxyribonuclease I: 4 u/ml；	Tyrode solution	Atta, H., Gheinani, A., Wacker, A., Heshmati, Y., Bigger-Allen, A., Lambrinos, G., Gao, Y., Bielenberg, D. and Adam, R.: A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury., J Vis Exp , , 2020
小鼠	Mouse	尿路上皮 / Urothelial	Collagenase Type 1: 0.5%	PBS	Huang, C., Chen, C. and Shyr, C.: The Anti-Tumor Effect of Intravesical Administration of Normal Urothelial Cells on Bladder Cancer., Cytotherapy 19, 1233-1245, 2017
小鼠	Mouse	骨髓基質(zhì)細(xì)胞 / Bone marrow stromal	Collagenase Type 1: 0.2%	PBS	Ishida, T., Suzuki, S., Lai, C., Yamazaki, S., Kakuta, S., Iwakura, Y., Nojima, M., Takeuchi, Y., Higashihara, M., Nakauchi, H. and Otsu, M.: Pre-Transplantation Blockade of TNF-α-Mediated Oxygen Species Accumulation Protects Hematopoietic Stem Cells., Stem Cells 35, 989-1002, 2017
小鼠	Mouse	耳蝸 / Cochlear	Papain: 20 u/ml； Trypsin: 0.05%	DMEM	Kim, Y., Wang, S., Tymanskyj, S., Ma, L., Tao, H. and Zhang, L.: Dcc Mediates Functional Assembly of Peripheral Auditory Circuits., Sci Rep 6, 23799, 2016
小鼠	Mouse	上皮干細(xì)胞 / Epithelial stem	Collagenase: 2%	DMEM/F12	Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor., J Vis Exp , , 2014
小鼠	Mouse, 26-30 g	肌間叢 / Myenteric plexus	Collagenase Type 2: 0.13%	Neurobasal A	Smith, T., Grider, J., Dewey, W. and Akbarali, H.: Morphine Decreases Enteric Neuron Excitability via Inhibition of Sodium Channels., PLoS ONE 7, e45251, 2012
小鼠	Mouse, 10 week	滑膜間充質(zhì) / Synovial mesenchymal	Collagenase: 0.1%； Deoxyribonuclease I: 0.005%	DMEM	Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE 7, e45517, 2012