worthington 前列腺組織分離指南

發(fā)布者：艾美捷科技發(fā)布時間：2022-06-09

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原代細(xì)胞分離中，針對前列腺組織涉及到前列腺癌成纖維細(xì)胞，前列腺基質(zhì)細(xì)胞、前列腺上皮/干細(xì)胞、頰神經(jīng)節(jié)，前列腺上皮/干細(xì)胞等等的解離通常會使用酶，如何選擇合適的酶來完成對應(yīng)前列腺組織的解離，以及原代細(xì)胞采集的常規(guī)操作， 艾美捷針對前列腺相關(guān)的組織分類解離分種屬、不同的細(xì)胞、酶組合推薦，推薦濃度、培養(yǎng)基以及參考文獻(xiàn)，詳細(xì)匯總指南：前列腺組織分離指南.png

本技術(shù)指南描述了標(biāo)準(zhǔn)實驗室程序；為建立細(xì)胞分離方案提供了一種合乎邏輯的實驗方法；并列出了許多組織特異性參考資料，以幫助開發(fā)有效的方法。更多其他組織的解離指南，點擊查看：各組織分離指南。

前列腺
種屬	種屬詳情	細(xì)胞名稱	酶組合推薦，含推薦濃度	培養(yǎng)基	參考文獻(xiàn)
人	Human	前列腺癌成纖維細(xì)胞 / Prostatic cancer fibroblasts	Collagenase: 225 u/ml； Hyaluronidase: 125 u/ml	RPMI 1640	Taylor, R., Toivanen, R., Frydenberg, M., Pedersen, J., Harewood, L., Collins, A., Maitland, N. and Risbridger, G.: Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status., Stem Cells 30, 1087, 2012
人	Human, 52-56 yr	前列腺基質(zhì)細(xì)胞 / Prostatic stromal cells	Collagenase Type 1: 0.2%	DMEM/F-12	Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R: DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells, Am J Physiol/Endo 290, E952-60, 2006
人	Human	前列腺基質(zhì)細(xì)胞 / Prostate stromal cells	Collagenase Type 1: 0.1%	RPMI 1640	Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi: Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R, Am J Pathol 165, 533-40, 2004
人	Human, fetal	前列腺成纖維細(xì)胞 / Prostatic fibroblasts	Collagenase Type 1: 0.125%	DMEM/F12	Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A: Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts, Endocrinology 139, 4672-8, 1998
小鼠	Mouse, 6-8 week	前列腺上皮/干細(xì)胞 / Prostate epithelial/stem	Collagenase Type 2: 0.5%； Trypsin: 0.05%	HBSS	Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells., Stem Cells 27, 2220-8, 2009
小鼠	Mouse, 2 week	前列腺上皮 / Prostatic epithelial	Collagenase Type 3: 170 u/ml	DMEM	Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake: Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate, Proc Natl Acad Sci U S A 101, 9375-80, 2004
小鼠	Mouse, male	前列腺干細(xì)胞 / Prostatic stem	Collagenase Type 1: 170 u/ml	DMEM	Dubey P, Wu H, Reiter RE, Witte ON: Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression, Cancer Res 61, 3256-61, 2001