HMGB1是在大腸桿菌中通過表達質(zhì)粒產(chǎn)生的，該質(zhì)粒編碼人類蛋白（Müller等人。HMGB1與雙鏈DNA相互作用的熱力學。生物化學2001, 40: 10254-61）。

艾美捷 HMGBiotech-HMGB1用于生物化學（#HM-100、HM-101）相關(guān)參數(shù)：

它具有以下序列：

“MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE”

分子質(zhì)量：HMGB1由215個氨基酸殘基組成，計算出的分子質(zhì)量約為24.8 kDa。在SDS-PAGE凝膠中，它的遷移位置大約在30 kD，可能是因為它含有不尋常數(shù)量的帶正電荷的氨基酸。

純度：純化的蛋白>95%均一（電泳和質(zhì)譜分析）。

活性：這種制劑具有較低的細胞因子活性，但作為DNA結(jié)合蛋白或抗原是完全活性的。

緩沖液：50 mM HEPES緩沖液pH 7.9，500 mM NaCl，0.5 mM DTT。

儲存：我們建議將產(chǎn)品在-20°C下冷凍保存。

本產(chǎn)品僅供研究使用，不能用于人體。

HMGBiotech-HMGB1用于生物化學部分出版參考：

Association of High Mobility Group Box-Protein 1 and Platelet Microparticles in Patients After Hematopoietic Stem Cell Transplantation

HMGB1 Release Induced by EV71 Infection Exacerbates Blood-Brain Barrier Disruption via VE-cadherin Phosphorylation

[Altered expression of 15-hydroxyprostaglandin dehydrogenase in chronic rhinosinusitis with nasal polyps]

HMGB1 induces hepcidin upregulation in astrocytes and causes an acute iron surge and subsequent ferroptosis in the postischemic brain

HMGB1-activated tumor-associated macrophages promote migration and invasion via NF-κB/IL-6 signaling in oral squamous cell carcinoma

The Expression of High Mobility Group Box-1 (HMG1) in the Peripheral Blood and Its Relation with Systemic Vasculitis Patients

The translational potential of miR-26 in atherosclerosis and development of agents for its target genes ACC1/2, COL1A1, CPT1A, FBP1, DGAT2, and SMAD7

The Anti-inflammatory Effects of HMGB1 Blockades in a Mouse Model of Cutaneous Vasculitis

HMGB1 cleavage by complement C1s and its potent anti-inflammatory product

HMGB1 Promotes the Release of Sonic Hedgehog From Astrocytes

艾美捷科技是HMGBiotech的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務。