worthington 胸腺組織分離指南

發(fā)布者：艾美捷科技發(fā)布時間：2022-06-10

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原代細胞分離中，針對胸腺涉及到樹突狀，胸腺基質細胞、脂肪干細胞、基質細胞，上皮，胸腺等等的解離通常會使用酶，如何選擇合適的酶來完成對應胸腺的解離，以及原代細胞采集的常規(guī)操作， 艾美捷針對胸腺相關的組織分類解離分種屬、不同的細胞、酶組合推薦，推薦濃度、培養(yǎng)基以及參考文獻，詳細匯總指南：胸腺組織分離指南.png

本技術指南描述了標準實驗室程序；為建立細胞分離方案提供了一種合乎邏輯的實驗方法；并列出了許多組織特異性參考資料，以幫助開發(fā)有效的方法。更多其他組織的解離指南，點擊查看：各組織分離指南。

胸腺
種屬	種屬詳情	細胞名稱	酶組合推薦，含推薦濃度	培養(yǎng)基	參考文獻
人	Human	樹突狀 / Dentritic	Collagenase Type 2: 0.1%； Deoxyribonuclease I: 0.002%	RPMI 1640	Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K: Human thymus contains 2 distinct dendritic cell populations, Blood 97, 1733-41, 2001
小鼠	Mouse	胸腺基質細胞 / Thymic stromal	Collagenase Type 4: 0.02%； Neutral Protease: 0.02%； Deoxyribonuclease I: 0.0025%	RPMI-1640	Rode, I. and Boehm, T.: Regenerative Capacity of Adult Cortical Thymic Epithelial Cells., Proc Natl Acad Sci U S A 109, 3463-8, 2012
小鼠	Mouse	基質細胞 / Stromal	Collagenase Type 3: 0.2%； Hyaluronidase: 0.1%	RPMI 1640	Phillips, Joy, Brondstetter, T., English, C., Lee, H., Virts, E, and Thoman, M.: IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution, J Immunol , 4869, 2004
小鼠	Mouse	樹突狀 / Dentritic	Collagenase Type 3: 0.1%； Deoxyribonuclease I: 325 u/ml	RPMI 1640	Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F, and Gabrieli, L: ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells, J Exp Med 196, 1415, 2002
小鼠	Mouse, 3-6 week	胸腺 / Thymic	Collagenase Type 3: 100-400 u/ml	HBSS	Smith KM, Olson DC, Hirose R, Hanahan D: Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance, Int Immunol 9, 1355-65, 1997
小鼠	Mouse (BALB/c, C3H, C57BL/6), 1-28 day	上皮，胸腺 / Epithelial, thymus	Neutral Protease: 1.5 痢/ml	DMEM	Ropke, C., van Deurs, B., and Petersen, O.: Short-term Cultivation of Murine Thymic Epithelial Cells in a Serum-Free Medium, In Vitro Cell Dev Biol 26, 671, 1990
小鼠	Mouse, C3H, 16 wk old, female	上皮 / Epithelial	Collagenase Type 3: 0.1%	DMEM	Ehmann, U., Shiurba, R., and Peterson, W.: Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture, In Vitro Cell Dev Biol 22 (12), 738, 1986
小鼠	Mouse, Swiss, 6 wk	胸腺 / Thymus	Collagenase Type 3: 150 u/ml	DMEM	Jones, K. and Pierre, R.: Analysis of Cellular Heterogeneity in Mouse Thymus Cultures, In Vitro 17, 431, 1981
大鼠	Rat, postnatal	胸腺 / Thymic	Trypsin: 0.05%	HBSS	Bonfanti, P., Claudinot, S., Amici, A., Farley, A., Blackburn, C. and Barrandon, Y.: Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells., Nature 466, 978, 2010
大鼠	Rat	胸腺上皮 / Thymic epithelial	Collagenase Type 3: 0.1%	DMEM	Masuda, A and Matsuyama, M: Epithelial Cell Lines From Rat Thymoma and Rat Thymus,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 21C:4.1, 1995
大鼠	Rat (ACI/NMs X BUF/Mna) F1, male, 28 months ；Rat, (ACI/MNs) male, 8 weeks (also bovine, adult)	上皮 / Epthelial	Collagenase Type 3: 0.1%	Eagle's MEM Serum-free	Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990