活性氧(Reactiveoxygenspecies,ROS)主要包括羥基自由基、超氧自由基和過氧化氫。在細(xì)胞或組織的正常生理代謝過程中會(huì)產(chǎn)生活性氧，同時(shí)一些環(huán)境因子例如紫外照射、吸煙、環(huán)境污染等也可以誘導(dǎo)活性氧的產(chǎn)生。活性氧在體內(nèi)的失衡，將會(huì)導(dǎo)致細(xì)胞脂、蛋白和DNA的氧化損傷，繼而導(dǎo)致各種腫瘤、風(fēng)濕性關(guān)節(jié)炎、糖尿病、肝損傷、以及中樞神經(jīng)系統(tǒng)疾病等。

當(dāng)然，機(jī)體中也存在許多種抗氧化的物質(zhì)，包括抗氧化大分子、抗氧化小分子和酶等，能夠清除體內(nèi)產(chǎn)生的各種活性氧，以阻止活性氧造成的氧化損傷。一個(gè)體系內(nèi)的各種抗氧化大分子、抗氧化小分子和酶的總的水平即體現(xiàn)了該體系內(nèi)的總抗氧化能力。因此測(cè)定樣本中的總抗氧化能力（TAC）具有非常重要的生物學(xué)意義。

作為專業(yè)的生命科學(xué)醫(yī)藥原料供應(yīng)商，Cell Biolabs中國區(qū)金牌代理，艾美捷科技為您推薦：適用于組織裂解液，細(xì)胞裂解液，血清，血漿（檸檬酸鹽  ），尿液，食物提取物的，OxiSelect 總抗氧化能力(TAC)分析試劑盒

Fig. 試劑盒原理圖

* 本產(chǎn)品僅適用于科研用途.

FAQ常見問題：

1.總抗氧化能力（TAC）檢測(cè)試劑盒，有物種種屬限制嗎？

我們的總抗氧化能力（TAC）檢測(cè)試劑盒，適用于任何生物蛋白質(zhì)樣品。因?yàn)樵噭┖性聿⒉灰蕾囉诳赡芤蛭锓N而異的蛋白質(zhì)結(jié)構(gòu)。

2.是否可以檢測(cè)凍存過的樣本？

我們的總抗氧化能力（TAC）檢測(cè)試劑盒，通常適用于在 -80?C 下保存兩個(gè)月以內(nèi)樣品，但我們建議盡可能測(cè)試新鮮樣品以獲得最佳結(jié)果。

3.在制備食品樣品時(shí)，1:2, w/v 是什么意思？

食品樣品應(yīng)準(zhǔn)備為 1 g 樣品：2 mL 去離子水。

4.檸檬酸鹽血漿樣品可以用于該測(cè)定嗎？

檸檬酸鹽血漿樣品與總抗氧化能力測(cè)定兼容。 但應(yīng)避免使用EDTA，因?yàn)檫@會(huì)干擾檢測(cè)。

5.該試劑盒可以測(cè)量細(xì)胞培養(yǎng)上清液的總抗氧化能力嗎？

不幸的是，我們的總抗氧化能力測(cè)定與細(xì)胞培養(yǎng)上清液不兼容。該測(cè)定基于銅的還原，介質(zhì)中存在的鐵會(huì)干擾該反應(yīng)。

結(jié)果展示：

近期發(fā)表文章：

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