The Plasmid midiPREP Kit provides a fast, simple, and cost-effective plasmid midiprep method for isolating the plasmid DNA from the cultured bacterial cells. The Plasmid midiPREP Kit is based on the alkaline lysis of bacterial cells, followed by binding DNA onto the glass fiber matrix of the spin column in the presence of high salt. Phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA is eluted in a small volume of the Tris buffer (included in each kit) or water (pH is between 7.0 and 8.5). The plasmid DNA purified with the Plasmid midiPREP Kit is suitable for a variety of routine applications, including the restriction enzyme digestion, sequencing, library screening, in vitro translation, transfection of robust cells, ligation, and transformation. The entire procedure can be completed within 40 minutes.
產(chǎn)品特點
此試劑盒提供了一種從培養(yǎng)的細菌中快速、簡單分離質(zhì)粒DNA的方法. 先使細菌細胞壁鹼性裂解,后將 DNA 于高鹽環(huán)境經(jīng)離心與管柱的玻璃纖維結(jié)合. 提取的DNA可使用在限制酶作用,定序,細胞轉(zhuǎn)殖等實驗上. 整個提取程序可在 40 分鐘內(nèi)完成.