- 中文名稱
B-Cell Activating Factor (BAFF) BioAssay ELISA Kit (Mouse)
- 英文名字
- B-Cell Activating Factor (BAFF) BioAssay ELISA Kit (Mouse)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- 023645
- 產(chǎn)品報(bào)價(jià)
- ￥1/48Tests ￥1/96Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- The Mouse B-cell Activating Factor (BAFF) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of BAFF in mouse serum, plasma and other biological fluids.Detection Range:62.5-4,000pg/mlSensitivity:< 28.4pg/mlPrecision:Intra-Assay: CV<10%Inter-Assay: CV<12%Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BAFF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BAFF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BAFF in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*023645A: Microtiter Plate, 1x96 wells, Pre-coated; ready to use*023645B: Standard, 2x1vial 023645C: Standard Diluent, 1x20ml*023645D: Detection Reagent A, 1x120ul *023645E: Detection Reagent B, 1x120ul 023645F: Assay Diluent A, 1x12ml023645G: Assay Diluent B, 1x12ml023645H: TMB Substrate, 1x9ml023645K: Stop Solution, 1x6ml023645L: Wash Buffer, 30x, 1x20ml Storage and Stability:Store *023645A, *023645B, *023645D and *023645E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
- 產(chǎn)品特點(diǎn)
- 針對(duì)B-Cell Activating Factor (BAFF) BioAssay ELISA Kit (Mouse)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到B-Cell Activating Factor (BAFF) BioAssay ELISA Kit (Mouse)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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