Cell Division Cycle Protein 25 (CDC25) BioAssay ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDC25 in human serum, plasma, tissue homogenates and other biological fluids.Detection Range:0.156-10ng/mlSensitivity:0.063ng/mlIntra-Assay CV:<10% Inter-Assay CV:<12%Kit Components:*024049A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*024049B: Standard, 2x1vial024049C: Standard Diluent, 1x20ml*024049D: Detection Reagent A, 1x120ul*024049E: Detection Reagent B, 1x120ul024049F: Assay Diluent A, 1x12ml024049G: Assay Diluent B, 1x12ml024049H: TMB Substrate, 1x9ml024049K: Stop Solution, 1x6ml024049L: Wash Buffer, 30X, 1x20mlPrecaution:The Stop Solution (024049K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *024049A, *024049B, *024049D and *024049E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
產(chǎn)品特點
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保存建議
建議收到Cell Division Cycle Protein 25 (CDC25) BioAssay ELISA Kit (Human)產(chǎn)品后將其置于4°C/-20°C保存。