- 中文名稱
Insulin (INS) BioAssay ELISA Kit (Rat)
- 英文名字
- Insulin (INS) BioAssay ELISA Kit (Rat)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- 025827
- 產(chǎn)品報(bào)價(jià)
- ￥1/48Tests ￥1/96Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Insulin (INS) BioAssay ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of INS in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Principle of the Test:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for INS has been pre-coated onto the wells of the provided microtiter strips. A competitive inhibition reaction occurs between biotin-labeled INS and unlabeled INS (standards or samples) with the pre-coated antibody specific for INS. After incubation, the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of INS in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of INS in the sample.Detection Range:123.5-10,000pg/mlSensitivity:52.4pg/ml Intra-Assay CV:<10%Inter-Assay CV:<12%Kit Components:*025827A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*025827B: Standard, 2x1 vial025827C: Standard Diluent, 1x20ml*025827D: Detection Reagent A (green), 1x1vial*025827E: Detection Reagent B (red), 1x120ul025827F: Assay Diluent A, 1x12ml025827G: Assay Diluent B, 1x12ml025827H: TMB Substrate, 1x9ml*025827J: Positive Control 1393.3pg/ml, 1x1vial025827K: Stop Solution, 1x6ml025827L: Wash Buffer, 30X, 1x20ml025827M: Detection Reagent A Diluent, 1x300ulPrecaution:The Stop Solution (025827K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *025827A, *025827B, *025827D, *025827E and *025827J at -20°C. After reconstitution of *025827J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Notes on 025827J - Positive Control:Intended Use:The control vials are from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.Characteristics:The control vials contain rat insulin in rat serum (lyophilized powder). Note: The rat based serum used in this product are from healthy rat of SPF standard (specific pathogen-free animals).Expected Value:1393.3pg/mlUsage: Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 50ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.7. Add 50ul Stop Solution. Read at 450nm immediately.
- 產(chǎn)品特點(diǎn)
- 針對(duì)Insulin (INS) BioAssay ELISA Kit (Rat)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Insulin (INS) BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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