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細(xì)胞分析試劑盒
中文名稱

Klotho (KL) BioAssay ELISA Kit (Rat)

英文名字
Klotho (KL) BioAssay ELISA Kit (Rat)
供應(yīng)商
USBiological
產(chǎn)品貨號
026398
產(chǎn)品報(bào)價(jià)
¥1/48Tests   ¥1/96Tests
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
Klotho (KL) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KL in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:0.312-20ng/mlSensitivity:0.118ng/mlIntra-Assay: CV: <10% Inter-Assay: CV: <12%Kit Components:*026398A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use*026398B: Standard, 2x1 vial026398C: Standard Diluent, 1x10ml or 1x20ml*026398D: Detection Reagent A (green), 1x60ul or 1x120ul*,026398E: Detection Reagent B (red), 1x60ul or 1x120ul 026398F: Assay Diluent A, 1x6ml or 1x12ml026398G: Assay Diluent B, 1x6ml or 1x12ml026398H: TMB Substrate, 1x9ml*026398J: Positive Control, 1.38ng/ml, 1x1 vial026398K: Stop Solution, 1x3ml or 1x6ml026398L: Wash Buffer, 30X, 1x10ml or 1x20mlPrecaution:The Stop Solution (026398K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *026398A, *026398B, *026398D, *026398E and *026398J at -20°C. After reconstitution of *026398J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Notes on 026398J: Positive Control:Intended Use:The control vial is from a rat serum sample known to contain KL. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.Characteristics:The control vial contains rat KL in rat serum (lyophilized powder). Note: The rat source material used in this product is from healthy animal of SPF standard (specific pathogen-free animals).Expected Values:1.38ng/mlUsage: Dissolve with 150ul standard dilutent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
產(chǎn)品特點(diǎn)
針對Klotho (KL) BioAssay ELISA Kit (Rat)該產(chǎn)品的特點(diǎn)優(yōu)勢,歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
保存建議
建議收到Klotho (KL) BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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