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細(xì)胞分析試劑盒
中文名稱(chēng)

Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) BioAssay ELISA Kit (Human)

英文名字
Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) BioAssay ELISA Kit (Human)
供應(yīng)商
USBiological
產(chǎn)品貨號(hào)
026928
產(chǎn)品報(bào)價(jià)
￥1/48Tests   ￥1/96Tests
產(chǎn)品說(shuō)明書(shū)
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USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶(hù)，幫助客戶(hù)，且擁有獨(dú)立的專(zhuān)業(yè)銷(xiāo)售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷(xiāo)團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢(xún)響應(yīng)，專(zhuān)業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
產(chǎn)品描述
The Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MCL1 in human tissue homogenates, cell lysates and other biological fluids.Detection Range:0.312-20ng/mlSensitivity:0.127ng/mlPrecision:Intra-Assay %CV: <10%Inter-Assay %CV: <12%Kit Components:*026928A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated; ready to use*026928B: Standard, 2x1vial 026928C: Standard Diluent, 1x10ml or 1x20ml*026928D: Detection Reagent A, 1x60ul or 1x120ul *026928E: Detection Reagent B, 1x60ul or 1x120ul 026928F: Assay Diluent A, 1x6ml or 1x12ml026928G: Assay Diluent B, 1x6ml or 1x12ml026928H: TMB Substrate, 1x4.5ml or 1x9ml*026928J: Positive Contro, 2.81ng/ml, 1x1vial026928K: Stop Solution, 1x3ml or 1x6ml026928L: Wash Buffer, 30X, 1x10ml or 1x20ml Precaution:The Stop Solution (026928K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *026928A, *026928B, *026928D, *026928E and *026928J at -20°C. After reconstitution of *026928J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Notes on 026928J: Positive ControlIntended Use:The positive control is from a human serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.Characteristics:The positive control vial contains human MCL1 in human serum (lyophilized powder). Note: The human based material used in this product is screened for HIV, Hepatitis A, B, C, HTLV and Syphilis.Expected Values:2.81ng/mlUsage: Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.
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保存建議
建議收到Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) BioAssay ELISA Kit (Human)產(chǎn)品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶(hù)提供了諸多超值選擇。
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