Osteoprotegerin (OPG) BioAssay ELISA Kit (Rat)-Osteoprotegerin (OPG) BioAssay ELISA Kit (Rat)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Osteoprotegerin (OPG) BioAssay ELISA Kit (Rat)

英文名字: Osteoprotegerin (OPG) BioAssay ELISA Kit (Rat)

供應(yīng)商: USBiological

產(chǎn)品貨號: 027264

產(chǎn)品報價: ￥1/48Tests ￥1/96Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Osteoprotegerin (OPG) BioAssay ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of OPG in rat serum, plasma, tissue homogenates and other biological fluids.Detection Range:0.156-10ng/mlSensitivity:0.055ng/mlIntra-Assay CV:<10% Inter-Assay CV:<12%Kit Components:*027264A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use*027264B: Standard, 2x1 vial027264C: Standard Diluent, 1x20ml*027264D: Detection Reagent A (green), 1x120ul*027264E: Detection Reagent B (red), 1x120ul027264F: Assay Diluent A, 1x12ml027264G: Assay Diluent B, 1x12ml027264H: TMB Substrate, 1x9ml*027264J: Positive Control, 0.87ng/ml, 1x1 vial027264K: Stop Solution, 1x6ml027264L: Wash Buffer, 30X, 1x20mlPrecaution:The Stop Solution (027264K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.Storage and Stability:Store *027264A, *027264B, *027264D, *027264E and *027264J at -20°C. After reconstitution of *027264J, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Notes on 027264J: Positive ControlIntended Use:The positive control is from a rat serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly.Characteristics:The positive control vial contains rat OPG in rat serum (lyophilized powder). Note: The rat based serum used in this product is from healthy rat of SPF standard (specific pathogen-free animals).Expected Values:0.87ng/mlUsage: Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20°C. After reconstitution, store Positive Control vial at 4°C and use within 5 days.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.8. Add 50ul Stop Solution. Read at 450nm immediately.

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保存建議: 建議收到Osteoprotegerin (OPG) BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C/-20°C保存。

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