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細胞分析試劑盒
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中文名稱

Progranulin (human) BioAssay ELISA Kit (Proepithelin, PEPI, PC Cell-derived Growth Factor)

英文名字
Progranulin (human) BioAssay ELISA Kit (Proepithelin, PEPI, PC Cell-derived Growth Factor)
供應(yīng)商
USBiological
產(chǎn)品貨號
045167
產(chǎn)品報價
¥1/96Tests
產(chǎn)品說明書
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產(chǎn)品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨立的專業(yè)銷售團隊、技術(shù)支持團隊、市場營銷團隊、進出口報關(guān)團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. New cutting-edge publications have shown that progranulin might be a biomarker not only for FTLD, but also for other types of Alzheimer's disease (AD). This new data even supposes the potential of progranulin to be a biomarker for MCI (Mild Cognitive Impairment), which would be a hallmark in AD research. Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.Sample Type:Cell Culture Supernatant, Plasma, Serum, Urine Intended Use:The Progranulin (human) ELISA Kit is to be used for the in vitro quantitative determination of human progranulin in serum, plasma, urine, cell culture supernatant and cerebrospinal fluid (CSF; R. Ghidoni et al.; Neurology 71, 1235 (2008)). Sensitivity:32pg/mlRange:0.063-4ng/ml Specificity:Detects human progranulin. Test Principle:This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.Kit Components:Coated plate with human progranulin Antibody , 1x1Wash Buffer 10X, 2x30mlELISA Buffer 10X, 2x30mlDetection Antibody, 1x30ulHRP Labeled Streptavidin (lyophilized), 1x2ughuman progranulin Standard (lyophilized), 1x8ng TMB Substrate Solution, 1x12mlStop Solution, 1x12ml2 plate sealers (plastic film) 2 silica Gel MinibagsStorage and Stability:Store *xxx powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產(chǎn)品特點
針對Progranulin (human) BioAssay ELISA Kit (Proepithelin, PEPI, PC Cell-derived Growth Factor)該產(chǎn)品的特點優(yōu)勢,歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
保存建議
建議收到Progranulin (human) BioAssay ELISA Kit (Proepithelin, PEPI, PC Cell-derived Growth Factor)產(chǎn)品后將其置于+4°C/+4°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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