Acyl-coenzyme A: cholesterol acyltransferase-1 -ACAT-1) catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A, and may play a role in the development of atherosclerosis. ACAT-1 is ubiquitously expressed, with highest levels observed in sebaceous glands, steroidogenic tissues, and macrophages. Human ACAT-1 has 550 amino acids and an estimated molecular weight of 65 kDa. Cayman s affinity-purified antibody recognizes a 50 kDa band in most cultured cell lines as reported in the literature. In addition, the antibody reacts with a native form of ACAT-1 at about 65 kDa in liver tissues. Cayman Chemical s affinity-purified ACAT-1 polyclonal antibody can be used for western blot analysis of ACAT-1 from samples of human, murine, rat, and porcine origin. Other applications for use of this antibody have not yet been tested.
應(yīng)用類型
WB
免疫原
human ACAT-1 amino acids 6-23 -KMSLRNRLSKSRENPEED)
來源宿主
rabbit
反應(yīng)性
Human ACAT-1+|Mouse ACAT-1+|Porcine ACAT-1+|Rat ACAT-1+