Two types of 15-LO have been discovered and characterized, both of which metabolize arachidonic acid -AA) to produce 15-S)-hydroxyeicosatetraenoic acid -15-S)-HETE). 15-LO-1 oxygenates AA at both C15 and C12, whereas 15-LO-2 exclusively oxygenates C15 of AA. Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1. Expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea and may play a role in the normal development of these tissues. The protein levels and enzymatic activity of 15-LO-2 are both down-regulated in prostate cancer compared with normal and benign prostate tissues, implicating a possible protective role for 15-LO-2 against tumor formation. Cayman Chemical s 15-LO-2 polyclonal antibody can be used for western blot analysis for 15-LO-2 on samples of human origin. Other applications for use of this antibody have not yet been tested.
應(yīng)用類型
WB
免疫原
human 15-lipoxygenase-2 -15-LO-2) amino acids 161-179 -CLDEKTVEDLELNIKYSTA)