The cellular trafficking of arachidonoyl ethanolamide -anandamide; AEA) by neurons is dominated by transport and/or hydrolysis by fatty acyl amide hydrolase -FAAH) within the neurons. CAY10455 is a labeled analog of AEA that is non-fluorescent when outside the cell. Upon transport into the cell interior, it is cleaved by esterases to give a bright fluorescence at 530 nm. CAY10455 uptake into C6 glioma cells is inhibited by AEA and its analogs, and conversely CAY10455 inhibits the uptake of tritiated AEA, indicating that they compete for the AEA transporter.
產(chǎn)品描述
A labeled analog of AEA that is non-fluorescent when outside the cell but upon transport into the cell, is cleaved by esterases to give a bright fluorescence at 530 nm; uptake into C6 glioma cells is inhibited by AEA and its analogs, and conversely CAY10455 inhibits the uptake of tritiated AEA, indicating that they compete for the AEA transporter