Biosynthesis of 12-R)-HETE in invertebrates is via lipoxygenation of arachidonic acid. In mammals, 12-R)-HETE can be produced by 12-R)-LOs and also by CYP450 oxidation. The activity of 12-R)-HETE in mammals is predominantly proinflammatory. 12-R)-HETE exhibits dose-dependent leukocyte chemotaxis at concentrations as low as 100 nM, and lowers intraocular pressure in rabbits.