Human Shh cDNA encodes a 462 amino acid (aa) residue (45 kDa) precursor protein with a 23 aa signal peptide. An autocatalytic cleavage reaction yields a 19 kDa (residues 24 - 197) amino-terminal fragment (Shh-N), and a 25 kDa (residues 198 - 462) carboxy-terminal domain (Shh-C). The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N. When Shh is expressed in insect or mammalian cells, a palmitoyl group is also attached to the N-terminal cysteine of Shh-N via an amide linkage.
應(yīng)用類型
WB
免疫原
該Anti-Mouse Sonic Hedgehog (Shh) C-terminal的詳細(xì)信息查看ReliaTech提供的產(chǎn)品說(shuō)明書(shū)。
來(lái)源宿主
該Anti-Mouse Sonic Hedgehog (Shh) C-terminal的詳細(xì)信息查看ReliaTech提供的產(chǎn)品說(shuō)明書(shū)。
反應(yīng)性
該Anti-Mouse Sonic Hedgehog (Shh) C-terminal的詳細(xì)信息查看ReliaTech提供的產(chǎn)品說(shuō)明書(shū)。
保存建議
該Anti-Mouse Sonic Hedgehog (Shh) C-terminal的詳細(xì)信息查看ReliaTech提供的產(chǎn)品說(shuō)明書(shū)。溶解建議:Centrifuge vial prior to opening. Reconstitute the antibody with 500 μl sterile PBS and the final concentration is 200 μg/ml.