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細(xì)胞分析試劑盒
中文名稱

PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)

英文名字
PDE4A, BioAssay Kit (cAMP-specific 3',5'-cyclic Phosphodiesterase 4A, DPDE2, PDE46, DPDE2)
供應(yīng)商
USBiological
產(chǎn)品貨號(hào)
137340
產(chǎn)品報(bào)價(jià)
¥1/96Tests
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USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4A is widely expressed in brain tumors and promotes their growth and its inhibitor Rolipram is identified as a potent anti- inflammatory agent. The PDE4A Assay Kit is designed for identification of PDE4A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4A to the binding agent.Intended Use:Designed for identification of PDE4A inhibitors using fluorescence polarization.Test Principle:The PDE4A inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE4A1 enzyme, fluorescently labeled PDE4A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.Kit Components:PDE4A1a: 1x300ngFAM-Cyclic-3’, 5’-AMP: 20uM: 1x50ulPDE assay buffer: 1x12mlBinding Agent: 1x100ulBinding Agent Diluent: 1x10mlBlack, low binding NUNC black microtiter plate: 1 plateStorage and Stability:Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
產(chǎn)品特點(diǎn)
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其他
Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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