- 中文名稱
BDNF BioAssay ELISA Kit (Rat)
- 英文名字
- BDNF BioAssay ELISA Kit (Rat)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- 143727
- 產(chǎn)品報(bào)價(jià)
- ￥1/96Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kD, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. Intended Use:BDNF BioAssay ELISA Kit is a sandwich ELISA for quantitative detection of rat BDNF in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate).Range:31.2pg/ml-2000pg/mlSensitivity:<15pg/mlSpecificity:Natural and recombinant rat BDNFCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Kit Components:143727A: Microtiter Strips, 1x96 wells143727B: BDNF Standard, 2x10ng143727C: Anti-rat BDNF (Biotin), 1x130ul (1:100 dilution)USB Cat NoKit Component143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability:Store *143727B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS or PBS.3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS or PBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 15-20 minutes.5. Add TMB stop solution and read.
- 產(chǎn)品特點(diǎn)
- 針對(duì)BDNF BioAssay ELISA Kit (Rat)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到BDNF BioAssay ELISA Kit (Rat)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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