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細胞分析試劑盒
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中文名稱

CD163 BioAssay ELISA Kit, Human

英文名字
CD163 BioAssay ELISA Kit, Human
供應商
USBiological
產(chǎn)品貨號
143759
產(chǎn)品報價
¥1/96Tests
產(chǎn)品說明書
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背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理,是行業(yè)中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業(yè)銷售團隊、技術(shù)支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應,專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務。我們致力于為您提供有價值的產(chǎn)品和服務,在意您的成功!
產(chǎn)品描述
CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.Uniprot ID:Q86VB7Entrez Gene ID:9332Intended Use:CD163 BioAssay ELISA Kit is a sandwich ELISA for quantitative detection of human CD163 in cell culture supernates, serum and plasma (heparin).Range:1.56-100ng/mlSensitivity:<150 pg/ml Specificity:Natural and recombinant human CD163Crossreactivity:No detectable crossreactivity with other relevant proteinsIntra-Assay CV(%): 4.2, 4.5,5.3Inter-Assay CV(%): 6.7, 5.4, 5.6Kit Components:143759A: Microtiter Strips, 1x96 wells*143759B: CD163 Standard, 2x1 vial143759C: Anti-human CD163 (Biotin), 1x130ulUSB Cat NoKit Component143696: Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (1:100 dilution)143697: Sample Diluent Buffer, 1x30ml143698: Antibody Diluent Buffer, 1x12ml143699: ABC Diluent Buffer, 1x12ml143700: TMB Color Developing Agent, 1x10ml143701: TMB Stop Solution, 2N H2SO4, 1x10mlStorage and Stability:Store *143759B powder at 4°C. Once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Assay Summary:1. Add samples and standards and incubate the plate at 37°C for 90 minutes. Do not wash. 2. Add biotinylated antibodies and incubate the plate at 37°C for 60 minutes. Wash plate 3 times with 0.01M TBS.3. Add ABC working solution and incubate the plate at 37°C for 30 minutes. Wash plate 5 times with 0.01M TBS.4. Add TMB color developing agent and incubate the plate at 37°C in dark for 25-30 minutes.5. Add TMB stop solution and read.
產(chǎn)品特點
針對CD163 BioAssay ELISA Kit, Human該產(chǎn)品的特點優(yōu)勢,歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
保存建議
建議收到CD163 BioAssay ELISA Kit, Human產(chǎn)品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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