Sandwich ELISA quantitative detection kit for Rat EGF in cell culture supernates, serum, plasma (heparin, EDTA), tissue homogenates and urine.Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.Assay Range:7.8pg/ml-500pg/mlSensitivity:< 1 pg/mlCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:P07522 Entrez Gene ID:25313Kit Components:96-well plate pre-coated with anti-EGF, RatLyophilized recombinant EGF, Rat standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10mlStorage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.