Sandwich ELISA quantitative detection kit for Rat GDNF in cell culture supernates, serum and plasma (heparin, EDTA).Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.1 GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.2 The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.Assay Range:31.2pg/ml-2000pg/mlSensitivity:< 4 pg/mlCrossreactivity:There is no detectable cross-reactivity with other relevant proteins.Uniprot ID:A7UGJ1Entrez Gene ID:25453Kit Components:96-well plate pre-coated with anti-GDNF, RatLyophilized recombinant GDNF, Rat standard, 10ng/tube, 2EaUSB Cat No Kit Component143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)143697 Sample diluent buffer, 30ml143698 Antibody diluent buffer, 12ml143699 ABC diluent buffer, 12ml143700 TMB color developing agent, 10ml143701 TMB stop solution, 10mlStorage and Handling:Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.