TNF-alpha, BioAssay? ELISApot Development Module (Rat) (Cachetin, DIF, TNF, tnfa, tnf-a, TNFSF1A, TNFSF2)-TNF-alpha, BioAssay? ELISApot Development Module (Rat) (Cachetin, DIF, TNF, tnfa, tnf-a, TNFSF1A, TNFSF2)代理/參數(shù)/廠家-生化試劑-艾美捷科技有限公司

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中文名稱: TNF-alpha, BioAssay? ELISApot Development Module (Rat) (Cachetin, DIF, TNF, tnfa, tnf-a, TNFSF1A, TNFSF2)

英文名字: TNF-alpha, BioAssay? ELISApot Development Module (Rat) (Cachetin, DIF, TNF, tnfa, tnf-a, TNFSF1A, TNFSF2)

供應(yīng)商: USBiological

產(chǎn)品貨號(hào): 145770

產(chǎn)品報(bào)價(jià): ￥1/1Kit

產(chǎn)品說明書: 點(diǎn)擊查看

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產(chǎn)品描述: Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types. Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21aa transmembrane segment, and a 177aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26kD type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55kD soluble trimeric form of TNF-alpha. TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-alpha regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-alpha is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.Sample Type:Whole cellsIntended Use:BioAssay? ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. Test Principle:The analyte-specific ELISpot Development Modules contain the components for analyte detection and visualization. Each module contains enough reagents for at least five 96-well microplates.Kit Components:Rat TNF-alpha Capture Antibody Concentrate, lyophilized, 1x vialRat TNF-alpha Detection Antibody Concentrate, lyophilized, 1x vialStorage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C for up to six months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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其他: Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。