Dihydro-Testosterone BioAssay ELISA Kit-Dihydro-Testosterone BioAssay ELISA Kit代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Dihydro-Testosterone BioAssay ELISA Kit

英文名字: Dihydro-Testosterone BioAssay ELISA Kit

供應(yīng)商: USBiological

產(chǎn)品貨號: 167550

產(chǎn)品報價: ￥1/96Tests

產(chǎn)品說明書: 點擊查看

購買方式: 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

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產(chǎn)品描述: 5a-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a class called androgens. DHT is a C19 steroid and possesses androgenic activity.The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione.The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Intended Use:The Dihydro- Testosterone ELISA kis is for the direct quantitative determination of Dihydrotestosterone by enzyme immunoassay in human serum. For in vitro diagnostic use only.Principle of the Test:The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of DHT in the sample. A set of standards is used to plot a standard curve from which the amount of DHT in patient samples and controls can be directly read.Detection Range: 0-2500 pg/mlSpecificity: 100%Sensitivity: 6pg/mlKit Components:Microtiter Plate, 1x96wellsDihydrotestosterone-Horseradish Peroxidase (HRP) Conjugate Concentrate, 1x200ulCalibrator A 0pg/ml, 1x2mlCalibrator B 25pg/ml, 1x600ulCalibrator C 100pg/ml, 1x600ulCalibrator D 500pg/ml, 1x600ulCalibrator E 1000pg/ml, 1x600ulCalibrator F 2500pg/ml, 1x600ulControl, 1x600ulWash Buffer Concentrate, 1x50mlAssay Buffer, 1x15mlTMB Substrate, 1x16mlStop Solution, 1x6mlStorage and Stability:Store all components at 4°C. Stable for 6 months. Reconstituted standards are stable for two weeks stored at 4oC. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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保存建議: 建議收到Dihydro-Testosterone BioAssay ELISA Kit產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。