Luciferase Reporter BioAssay Kit-Luciferase Reporter BioAssay Kit代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Luciferase Reporter BioAssay Kit

英文名字: Luciferase Reporter BioAssay Kit

供應(yīng)商: USBiological

產(chǎn)品貨號: 169702

產(chǎn)品報價: ￥1/200Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer.Sample type:Cell and Tissue culture homogenates (eukaryotic cells)Intended Use:Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.Test Principle:For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg2? and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg2? in an optimized buffer solution, USBio's Luciferase Reporter Assay Kit ensure maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples.Specificity:MammalianKit Components:Substrate A: 1 vial?Substrate B: 1 vialCell Lysis Buffer: 1 vialStorage and Stability:Store powder at 4°C, liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對Luciferase Reporter BioAssay Kit該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到Luciferase Reporter BioAssay Kit產(chǎn)品后將其置于-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。