Peroxidase Activity BioAssay Kit, Colorimetric/Fluorometric-Peroxidase Activity BioAssay Kit, Colorimetric/Fluorometric代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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產(chǎn)品描述: Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.Intended Use:The Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples.Sensitivity:The detection limit is 0.1mu/well via colorimetric or 0.01mu/well via fluorometric method, based on unit definition.Test Principle:In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red- fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lambdamax=570nm) or fluorometric methods (Ex/Em = 535/587nm). The assay is simple, direct, highly sensitive and high throughput-ready. Specificity:MammalianKit Components:Assay Buffer, 1x25mlProbe (in DMSO), 1x0.2mlH2O2 Substrate (0.88 M), 1x0.1ml HRP Positive Control, 1x1mlStorage and Stability:Store powder at 4°C, liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點(diǎn): 針對Peroxidase Activity BioAssay Kit, Colorimetric/Fluorometric該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到Peroxidase Activity BioAssay Kit, Colorimetric/Fluorometric產(chǎn)品后將其置于-20°C保存。

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