Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1) BioAssay ELISA Kit (Rat) discontinued-Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1) BioAssay ELISA Kit (Rat) discontinued代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1) BioAssay ELISA Kit (Rat) discontinued

英文名字: Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1) BioAssay ELISA Kit (Rat) discontinued

供應(yīng)商: USBiological

產(chǎn)品貨號: 185171

產(chǎn)品報價: ￥1/48Test ￥1/96Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1) BioAssay ELISA Kit is a 1.5-hour solid-phase competitive ELISA designed for the quantitative determination of CEACAM1 in rat serum, plasma, tissue homogenates and body fluids. This kit is intended for research use only and may not be used for therapeutic or diagnostic applications.Detection Range:250-5000pg/mlSensitivity:1pg/mlPrecision:Intra-assay %CV: <10Inter-assay %CV: <10Specificity: This assay has high sensitivity and excellent specificity for detection of CEACAM1 (Rat). No significant crossreactivity or interference with analogs was observed.?Note: Crossreactivity detection between the CEACAM1 and all analogs has not been determined and crossreaction may exist in some cases.Kit Components:185171A: Microtiter Strips, 1x48 wells or 1x96 wells185171B: Enzyme Conjugate, 1x3ml or 1x6ml185171C: Standard, 0pg/ml, 1x1 vial185171D: Standard, 250pg/ml, 1x1 vial185171E: Standard, 500pg/ml, 1x1 vial185171F: Standard, 1000pg/ml, 1x1 vial185171G: Standard, 2500pg/ml, 1x1 vial185171H: Standard, 5000pg/ml, 1x1 vial185171J: Substrate A, 1x6ml185171K: Substrate B, 1x6ml185171L: Stop Solution, 1x6ml185171M: Wash Solution, 100X, 1x10ml185171N: Balance Solution, 1x3mlNote:The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagent.? Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Assay Principle:This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate that has been pre-coated with a monoclonal anti-CEACAM1 antibody. The assay samples and standards are incubated together with CEACAM1-HRP conjugate for one hour. A competition for limited antibody binding sites on the plate occurs between CEACAM1-HRP conjugate and CEACAM1 in the samples and standards. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to terminate the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CEACAM1 concentration in the sample or standard. A standard curve is plotted relating the intensity of the color (O.D.) to the concentrations of the standards. The CEACAM1 concentration in each sample is interpolated from this standard curve.Contact:United States Biological4 Technology WaySalem, MA 01970www.usbio.netTech Support:?tech@usbio.netCustomer Service: service@usbio.netTel:?781-639-5092 ??800-520-3011Fax:?978-594-8052

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