Pyranose Oxidase catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars concomitant with the reduction of oxygen to hydrogen peroxide. It plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate hydrogen peroxide for the ligninolytic peroxidases, lignin peroxidase, and manganese-dependent peroxidase. The preferred substrate is D-glucose which is converted to 2-dehydro-D-glucose. It acts also on D-xylose, together with D-glucose the major sugars derived from wood, on L-sorbose, D-galactose and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in pentose phosphate pathway. It employs one cofactor, FAD.
應(yīng)用類(lèi)型
ELISA,Immunoprecipitation,Western Blot,
免疫原
Pyranose Oxidase [E.coli]
來(lái)源宿主
Goat
反應(yīng)性
Microbial
保存建議
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.