Localization of biotinylated probes with avidin produces may produce high background levels. Anti-biotin antibodies may be substituted for avidin to decrease non-specific binding.
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
來源宿主
Mouse
反應性
該【干冰】Anti-BIOTIN (MOUSE) Monoclonal Antibody - 200-301-098S的詳細信息查看Localization of biotinylated probes with avidin produces may produce high background levels. Anti-biotin antibodies may be substituted for avidin to decrease non-specific binding.提供的產(chǎn)品說明書。
保存建議
Store Biotin antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.