【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S-【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody

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中文名稱: 【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S

英文名字: 【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S

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背景資料: 6X His antibody is an Epitope tag which is a short peptide sequence that is easily recognized by tag-specific antibodies.? Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.?? Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.? This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.? This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.? The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.? Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

應(yīng)用類型: ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,

免疫原: This His antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

反應(yīng)性: 該【干冰】Anti-6X HIS EPITOPE TAG (MOUSE) Monoclonal Antibody - 200-301-382S的詳細(xì)信息查看6X His antibody is an Epitope tag which is a short peptide sequence that is easily recognized by tag-specific antibodies.? Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.?? Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.? This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.? This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.? The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.? Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.提供的產(chǎn)品說(shuō)明書。

保存建議: Store 6X His Tag Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

其他: Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。