- 中文名稱
【干冰】Anti-SMC1 pS957 (MOUSE) Monoclonal Antibody - 200-301-397
- 英文名字
- 【干冰】Anti-SMC1 pS957 (MOUSE) Monoclonal Antibody - 200-301-397
- 供應(yīng)商
- Rockland
- 產(chǎn)品貨號
- 200-301-397
- 產(chǎn)品報(bào)價(jià)
- ￥詢價(jià)/100ug
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 90%產(chǎn)品中國有現(xiàn)貨庫存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- Structural maintenance of chromosomes (SMC) proteins play important roles in sister chromatid cohesion, chromosome condensation, sex-chromosome dosage compensation, and DNA recombination and repair (DNA damage). Protein complexes containing heterodimers of the SMC1 and SMC3 proteins have been implicated specifically in both sister chromatid cohesion and DNA recombination.? ATM, a protein kinase belonging to the phosphatidylinositol 3-kinase family that regulates cell cycle checkpoints and DNA recombination and repair, phosphorylates SMC1 protein after ionizing irradiation. ATM protein kinase phosphorylates SMC1 on serines 957 and 966 in vitro and in vivo, and expression of an SMC1 protein mutated at these phosphorylation sites abrogates the ionizing irradiation-induced S phase cell cycle checkpoint.? Optimal phosphorylation of these sites in SMC1 after ionizing irradiation also requires the presence of the ATM protein kinase substrates NBS1 and BRCA1. These same sites in SMC1 are phosphorylated after treatment with UV irradiation or hydroxyurea in an ATM-independent manner, thus demonstrating that another kinase must be involved in responses to these cellular stresses. Yeast containing hypomorphic mutations in SMC1 and human cells overexpressing SMC1 mutated at both of these phosphorylation sites exhibit decreased survival following ionizing irradiation. These results demonstrate that SMC1 participates in cellular responses to DNA damage and link SMC1 to the ATM protein kinase signal transduction pathway.
- 應(yīng)用類型
- ELISA,Immunohistochemistry,IF Microscopy,Western Blot,
- 免疫原
- This antibody was produced from a synthetic peptide corresponding to aa 951-962 of human SMC1 by injection into a balb/c mouse.
- 來源宿主
- Mouse
- 反應(yīng)性
- H. sapiens (Human); Mus musculus (Mouse)
- 保存建議
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費(fèi)城郊區(qū)的農(nóng)場保有近20種實(shí)驗(yàn)動物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。
- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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