【干冰】Anti-HEF1 (aa 82-398) (MOUSE) Monoclonal Antibody - 200-301-904-【干冰】Anti-HEF1 (aa 82-398) (MOUSE) Monoclonal Antibody

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中文名稱: 【干冰】Anti-HEF1 (aa 82-398) (MOUSE) Monoclonal Antibody - 200-301-904

英文名字: 【干冰】Anti-HEF1 (aa 82-398) (MOUSE) Monoclonal Antibody - 200-301-904

供應(yīng)商: Rockland

產(chǎn)品貨號: 200-301-904

產(chǎn)品報(bào)價(jià): ￥詢價(jià)/100ug

產(chǎn)品說明書: 點(diǎn)擊查看

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背景資料: HEF1, also known as Enhancer of filamentation 1, CRK-associated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene.? HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation.? HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells.? Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.? HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas.?? HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1.? This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts, it is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells, it is localized predominantly in the cell periphery with particular concentration in lamellipodia, but it is also found in the nucleus.? HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue.? HEF1 is also detected in T-cells, B-cells and diverse cell lines.? HEF1 is activated upon induction of cell growth.? Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55.? Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle.? Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected.? Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle.

應(yīng)用類型: ELISA,IF Microscopy,Immunoprecipitation,Western Blot,

免疫原: Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein (hHEF1, 843 aa, predicted MW 92.8 kDa).

來源宿主: Mouse

反應(yīng)性: H. sapiens (Human); Mus musculus (Mouse); Rattus (Rat)

保存建議: Store vial at -20° C prior to opening.? Aliquot contents and freeze at -20° C or below for extended storage.? Avoid cycles of freezing and thawing.? Centrifuge product if not completely clear after standing at room temperature.? This product is stable for several weeks at 4° C as an undiluted liquid.? Dilute only prior to immediate use.

其他: Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費(fèi)城郊區(qū)的農(nóng)場保有近20種實(shí)驗(yàn)動物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。