【干冰】Anti-Ubiquitin (MOUSE) Monoclonal Antibody - 200-301-G61-【干冰】Anti-Ubiquitin (MOUSE) Monoclonal Antibody

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中文名稱: 【干冰】Anti-Ubiquitin (MOUSE) Monoclonal Antibody - 200-301-G61

英文名字: 【干冰】Anti-Ubiquitin (MOUSE) Monoclonal Antibody - 200-301-G61

供應(yīng)商: Rockland

產(chǎn)品貨號(hào): 200-301-G61

產(chǎn)品報(bào)價(jià): ￥詢價(jià)/200ug

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背景資料: Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity. The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate. Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBα and p27.

應(yīng)用類型: ELISA,Western Blot,

免疫原: Ubiquitin Antibody was produced in mice by repeated immunizations raised against native bovine ubiquitin conjugated to KLH.

來(lái)源宿主: Mouse

反應(yīng)性: Bovine (Bos Taurus); H. sapiens (Human); Mus musculus (Mouse); Rattus (Rat)

保存建議: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

其他: Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。