Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody Biotin Conjugated - 200-306-383-Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody Biotin Conjugated

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中文名稱: Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody Biotin Conjugated - 200-306-383

英文名字: Antibody for the detection of FLAG? conjugated proteins (MOUSE) Monoclonal Antibody Biotin Conjugated - 200-306-383

背景資料: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.? Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.?? Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.? This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.? This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.? The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.? Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.? Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG? and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

免疫原: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG? epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.

保存建議: Store vial at 4° C prior to restoration.?? For extended storage aliquot contents and freeze at -20° C or below.? Avoid cycles of freezing and thawing.? Centrifuge product if not completely clear after standing at room temperature.? This product is stable for several weeks at 4° C as an undiluted liquid.? Dilute only prior to immediate use.

其他: Rockland成立于1962年，是一家老牌抗體原產企業(yè)，其位于美國費城郊區(qū)的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。