A1AG serum protein, also called orosomucoid, is a monomer about 210aa residues long; the amino acid sequence has been determined through 192aa. A1AG is found in feline plasma in concentrations of 55-140mg per 100ml. It is a classical standard glycoprotein (40Kd, ~40% carbohydrates) for studies on the structure of the oligosaccharide units. Its biological significance is unknown, although it can bind progesterone 15x as strongly as albumin. Sialic-acid-deficient alpha-1-AG has an affinity for vitamin B-12. Clinically, alpha 1 acid glycoprotein is an acute-phase reactant that together with haptoglobin is an indicator of acute inflammation. The alpha 1 acid glycoprotein:haptolobin ratio is useful in studies of bone marrow disorders, hemolytic processes and metastases. It is a positive acute phase reactant, the levels of which increase significantly in cats in response to inflammation, tissue injury and disease. A1AGP is purified using a combination of acid extraction, precipitation, gel filtration and affinity chromatography steps.Source:Alpha-1-Acid Glycoprotein Protein, from rat plasmaApplications: Suitable for use in ELISA, Western Blot and Antibody Blocking. Other applications not tested.Recommended Dilution:Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.