Myoglobin is a single-chain heme containing protein with a molecular weight of ~17kD. It is the primary oxygen-carrying protein of muscle tissues. The same isoform is expressed in both cardiac and skeletal muscle. It is a sensitive biomarker for muscle injury and has been widely used as a marker of heart disease. Myoglobin is purified using a combination of gel-filtration and ion-exchange chromatography steps.Source:Myoglobin, from rat cardiac tissueMolecular Weight:~17kDApplications: Suitable for use in ELISA and Western Blot. Other applications not tested.Recommended Dilution:Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.