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中文名稱(chēng)

Interleukin-17A, Mouse, BioAssay ELISA Kit (IL-17A)

英文名字
Interleukin-17A, Mouse, BioAssay ELISA Kit (IL-17A)
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USBiological
產(chǎn)品貨號(hào)
216008
產(chǎn)品報(bào)價(jià)
￥1/96Tests
產(chǎn)品說(shuō)明書(shū)
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USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國(guó)區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶(hù)，幫助客戶(hù)，且擁有獨(dú)立的專(zhuān)業(yè)銷(xiāo)售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營(yíng)銷(xiāo)團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢(xún)響應(yīng)，專(zhuān)業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
產(chǎn)品描述
Mouse Interleukin-17A (mIL-17A), also know as CTLA-8, is a secreted, homodimeric glycoprotein linked by disulfide link with a molecular mass of about 35kD. The cytokine is the proto-type of a newly discovered pro-inflammatory cytokine family which consists of IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (IL-25) and IL-17F. IL-17A is mainly produced by a group of CD4 T helper cells termed Th17 cells.? In addition to Th17 cells, CD8 T helper cells, natural killer cells and granulocytes, can also secret the cytokine after proper stimulation. IL17A exhibits structural homology and cross-species bioactivity between human and mouse cells. ???IL-17A acts through its receptor IL-17R. It was found to stimulate the expression of pro-inflammatory cytokines including IL-6, MCP-1, G-CSF,GM-CSF,IL-1alpha and TNF-alpha.Most of those cytokines plays a role in the proliferation, maturation and chemotaxis of neutrophils.?Elevated levels of IL-17A have been found to implicate with autoimmune diseases, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer, vascular inflammation and development of atherosis.??This mouse IL-17A ELISA is a 3.5-hour solid phase immunoassay readily applicable to measure mouse IL-17A levels in cell culture supernatant and mouse serum in the range of 0 to 1000pg/ml. It showed no cross reactivity with other cytokines tested.?This mouse IL-17A ELISA is expected to be effectively used for further investigations into the relationship between IL-17A and the various conditions mentioned.?Intended Use:This mouse Interleukin-17A ELISA Kit is designed for the in vitro quantitative determination of mouse interleukin-17A (IL-17A) concentrations in cell culture supernatant and mouse serum.?Sensitivity:<2pg/mlRange:15.6-1000pg/mlTest Principle:This mouse IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to mouse IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for mouse IL-17A was added and incubated. Mouse IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound mouse IL-17A and other components in the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and aTMB(3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain mouse IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of mouse IL-17A in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus mouse IL-17A concentration (pg/ml). The concentration of IL-17A in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:ANTI-MOUSE IL-17A MICROTITER PLATE?BIOTIN CONJUGATE?AVIDIN CONJUGATE?MOUSE IL-17 STANDARD??CALIBRATOR DILUENT I?CALIBRATOR DILUENT II?WASH BUFFER (20X/96 wells, 30X/192 wells)??SUBSTRATE A??SUBSTRATE B??STOP SOLUTIONStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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保存建議
建議收到Interleukin-17A, Mouse, BioAssay ELISA Kit (IL-17A)產(chǎn)品后將其置于4°C/-20°C保存。
其他
Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶(hù)提供了諸多超值選擇。
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