The human BLC/BCA-1/CXCL13 BioAssay ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BLC. Sample Type:Serum, plasma, cell culture supernatants and urineIntended Use:Applications include: quantitative protein detection, establishing normal range and validation of antibody array results.Sensitivity:The minimum detectable concentration of BLC is <1.5pg/ml. Range:1.5-1000pg/mlSpecificity:Recognizes human BLC. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1beta, MIP-1delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. Test Principle:This assay employs an antibody specific for human BLC coated on a 96-well plate. Standards and samples are pipetted into the wells and BLC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BLC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BLC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.Kit Components:BLC Microplate (Item A), 1x96wellsWash Buffer Concentrate, 20X, (Item B), 1x25ml Standards (Item C), 2x vials Assay Diluent A (Item D), 1x30ml Assay Diluent B (Item E), 5X, 1x15ml Detection Antibody BLC (Item F), 2x vialsHRP-Streptavidin Concentrate (Item G), 200X, 1x200ul TMB One-Step Substrate Reagent (Item H), 1x12ml Stop Solution (Item I), 1x8mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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保存建議
建議收到BLC, BioAssay ELISA Kit (BCA-1, CXCL13, B Cell Attracting Chemokine 1, B Lymphocyte Chemoattractant) (Human)產(chǎn)品后將其置于-20°C保存。