VCAM-1, BioAssay ELISA Kit (Vascular Cell Adhesion Molecule-1, Cluster of Differentiation 106, CD106) (Mouse)-VCAM-1, BioAssay ELISA Kit (Vascular Cell Adhesion Molecule-1, Cluster of Differentiation 106, CD106) (Mouse)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: VCAM-1, BioAssay ELISA Kit (Vascular Cell Adhesion Molecule-1, Cluster of Differentiation 106, CD106) (Mouse)

英文名字: VCAM-1, BioAssay ELISA Kit (Vascular Cell Adhesion Molecule-1, Cluster of Differentiation 106, CD106) (Mouse)

供應商: USBiological

產(chǎn)品貨號: 220427

產(chǎn)品報價: ￥1/100Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: The mouse vascular cell adhesion molecule 1 (VCAM-1) or cluster of differentiation 106 (CD106) BioAssay ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. Sample Type:Serum and plasma (heparin, EDTA), cell culture supernatants. Intended Use:For use in quantitative protein detection, establishing normal range etc. Sensitivity:<10pg/mlRange:156-10,000pg/mlSpecificity:Recognizes natural and recombinant mouse vascular cell adhesion molecule 1. This ELISA kit shows no cross-reactivity with other relevant proteins.Test Principle:This assay employs a monoclonal antibody specific for mouse VCAM-1 coated on a 96-well plate. Standards and test samples are added to the wells and VCAM-1 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for VCAM-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VCAM-1 captured onto the plate.Kit Components:Microplate coated with anti-mouse monoclonal Ab against VCAM-1, 1x96 wells?Lyophilized recombinant mouse VCAM-1 standard (10ng/vial), 2x vials ?Biotinylated anti-mouse VCAM-1antibody, 1x130ul ?Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul ?Sample diluent buffer, 1x30ml ?Antibody diluent buffer, 1x12ml ?ABC diluent buffer, 1x12ml ?TMB color developing agent (Colorless), 1x10ml ?TMB stop solution, 1x10ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對VCAM-1, BioAssay ELISA Kit (Vascular Cell Adhesion Molecule-1, Cluster of Differentiation 106, CD106) (Mouse)該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到VCAM-1, BioAssay ELISA Kit (Vascular Cell Adhesion Molecule-1, Cluster of Differentiation 106, CD106) (Mouse)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。