Vascular Cell Adhesion Molecule 1, BioAssay ELISA Kit (Mouse) (VCAM-1, CD106, INCAM-100, CD106 Antigen)-Vascular Cell Adhesion Molecule 1, BioAssay ELISA Kit (Mouse) (VCAM-1, CD106, INCAM-100, CD106 Antigen)代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Vascular Cell Adhesion Molecule 1, BioAssay ELISA Kit (Mouse) (VCAM-1, CD106, INCAM-100, CD106 Antigen)

英文名字: Vascular Cell Adhesion Molecule 1, BioAssay ELISA Kit (Mouse) (VCAM-1, CD106, INCAM-100, CD106 Antigen)

供應(yīng)商: USBiological

產(chǎn)品貨號: 228011

產(chǎn)品報價: ￥1/48Tests ￥1/96Tests ￥1/24Tests

產(chǎn)品說明書: 點擊查看

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產(chǎn)品描述: This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.Sample Type:Serum, plasma, cell culture supernates and tissue homogenatesIntended Use:For the quantitative determination of mouse vascular cell adhesion molecule 1 (VCAM-1) concentrations in serum, plasma, cell culture supernates and tissue homogenates. Sensitivity:29.649pg/mlRange:39.062-2500pg/mlSpecificity:This assay has high sensitivity and excellent specificity for detection of mouse VCAM-1. No significant cross-reactivity or interference between mouse VCAM-1 and analogues was observed.Test Principle:This BioAssay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for VCAM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VCAM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VCAM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VCAM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components:Assay plate (12x8 coated Microwells), 1x96 wellsStandard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對Vascular Cell Adhesion Molecule 1, BioAssay ELISA Kit (Mouse) (VCAM-1, CD106, INCAM-100, CD106 Antigen)該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到Vascular Cell Adhesion Molecule 1, BioAssay ELISA Kit (Mouse) (VCAM-1, CD106, INCAM-100, CD106 Antigen)產(chǎn)品后將其置于4°C/-20°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。