LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)-LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)

英文名字: LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)

供應(yīng)商: USBiological

產(chǎn)品貨號: 298428

產(chǎn)品報價: ￥1/384Tests

產(chǎn)品說明書: 點擊查看

購買方式: 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

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產(chǎn)品描述: LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.Intended Use:The LSD1 BioAssay Kit is designed to measure LSD1 activity of purified LSD1 enzymes containing LSD1 using fluorimetry. The assay is based on the analysis of the hydrogen peroxide produced during the demethylation reaction mediated by LSD1. Test Principle:The key to the LSD1 BioAssay Kit is the methylated peptide substrate. Using this kit, only two simple steps on a microtiter plate are required for LSD1 reactions. First, a sample containing LSD1 is incubated in a reaction mixture with the methylated peptide substrate. Second, peroxidase and Amplex Red reagent are added and fluorescence (lambda ex= 530±13nm, lambda em= 590±18nm) is measured using a plate reader.Contraindications:DMSO >1%, strong acids or bases, ionic detergents, high saltApplications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:Black microtiter plate, 1x 384 wellsLSD1 recombinant enzyme, 1x60ug Methylated peptide substrate (200uM) 1x1ml Amplex Red reagent (10mM) 1x100ul Peroxidase (10U/ml) 1x200ul2x LSD1 assay buffer, 1x20ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產(chǎn)品特點: 針對LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)該產(chǎn)品的特點優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。

保存建議: 建議收到LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)產(chǎn)品后將其置于4°C/-20°C/-70°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。