- 中文名稱
Transforming growth Factor b1, BioAssay ELISA Kit (Canine) (TGF-b1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 Protein, Latency-Associated Peptide)
- 英文名字
- Transforming growth Factor b1, BioAssay ELISA Kit (Canine) (TGF-b1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 Protein, Latency-Associated Peptide)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- 351712
- 產(chǎn)品報(bào)價(jià)
- ￥1/48Tests ￥1/96Tests ￥1/24Tests
- 產(chǎn)品說明書
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- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。武漢艾美捷科技作為USBiological品牌中國區(qū)域總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立的專業(yè)銷售團(tuán)隊(duì)、技術(shù)支持團(tuán)隊(duì)、市場(chǎng)營銷團(tuán)隊(duì)、進(jìn)出口報(bào)關(guān)團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！
- 產(chǎn)品描述
- Transforming growth factor beta 1 or TGFbeta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. TGFbeta1 was first identified in human platelets as a protein with a molecular mass of 25kD with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390aa) that was proteolytically processed to produce a mature peptide of 112aa. TGFbeta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGFbeta1. Some T cells (e.g. regulatory T cells) release TGFbeta1 to inhibit the actions of other T cells. Interleukin 1- and interleukin 2-dependent proliferation of activated T cells, and the activation of quiescent helper T cells and cytotoxic T cells is prevented by the activity of TGFbeta1. Similarly, TGFbeta1 can inhibit the secretion and activity of many other cytokines including interferon-gamma, tumor necrosisfactor-alpha (TNF-alpha) and various interleukins. It can also decrease the expression levels of cytokine receptors, such as the IL-2 receptor to down-regulate the activity of immune cells. However, TGFbeta1 can also increase the expression of certain cytokines in T cells and promote their proliferation, particularly if the cells are immature. TGFbeta1 has similar effects on B cells that also vary according to the differentiation state of the cell. It inhibits proliferation and apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. The effects of TGFbeta1 on macrophages and monocytes are predominantly suppressive; this cytokine can inhibit the proliferation of these cells and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGFbeta1 can also have the opposite effect on cells of myeloid origin. For example, TGFbeta1 acts as a chemoattractant, directing an immune response to some pathogens; macrophages and monocytes respond to low levels of TGFbeta1 in a chemotactic manner. Furthermore, the expression of monocytic cytokines and phagocytic killing by macrophages can be increased by the action of TGFbeta1.Sample Type:Serum, plasma and cell culture supernatesIntended Use:This immunoassay kit allows for the in vitro quantitative determination of canine TGF-b1 concentrations in serum, plasma and cell culture supernates.Sensitivity:0.2ng/ml.Range:0.781-50ng/mlSpecificity:This assay recognizes canine TGF-beta1. No significantcross-reactivity or interference was observed.Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for TGF-beta1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. The concentration of TGF-beta1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:Assay plate, 1x plateStandard, 2x vialsSample Diluent, 1x20mlBiotin-antibody Diluent, 1x10mlHRP-avidin Diluent, 1x10mlBiotin-antibody, 1x120ulHRP-avidin, 1x120ulWash Buffer (25X), 1x20mlTMB Substrate, 1x10mlStop Solution, 1x10ml1N HCI, 1x10ml1.2N NaOH/0.5M HEPES, 1x10mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
- 針對(duì)Transforming growth Factor b1, BioAssay ELISA Kit (Canine) (TGF-b1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 Protein, Latency-Associated Peptide)該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Transforming growth Factor b1, BioAssay ELISA Kit (Canine) (TGF-b1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 Protein, Latency-Associated Peptide)產(chǎn)品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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